Excito-repellency and biological safety of beta-caryophyllene oxide against Aedes albopictus and Anopheles dirus (Diptera : Culicidae) Nararak, J. Di Giorgio, C. Sukkanon, C. Mahiou-Leddet, V. Ollivier, E. /Manguin, Sylvie Chareonviriyaphap, T. Excito-repellency test system beta-Caryophyllene oxide Phototoxic Genotoxic Mosquitoes The activity of beta-caryophyllene oxide as either a contact or noncontact repellent was evaluated against two laboratory strains (Aedes albopictus and Anopheles dirus) using an excito-repellency test system. N, N-Diethyl-3methylbenzamide (DEET) was used as a standard reference baseline for comparative purposes. beta-Caryophyllene oxide and DEET were tested at concentrations of 0.1, 0.25, 0.5 and 1.0% (v/v). In addition, the phototoxic and genotoxic effects of beta-caryophyllene oxide were investigated on Balb/c 3T3 mouse fibroblasts (3T3-L1) and Chinese hamster ovary cell line (CHO-K1). The results demonstrated that the higher concentrations of test compounds (0.5 and 1.0%) produced greater behavioral responses. Aedes albopictus was more sensitive to beta beta-caryophyllene oxide than An. dirus. Moderate avoidance response rates (25-56% escape) of Ae. albopictus at 0.5% and 1.0% beta-caryophyllene oxide were observed in contact and noncontact trials compared with low response rates from An. dirus (26-31% escape). DEET at <= 1% displayed lower irritancy and repellency (1-38%) than beta-caryophyllene oxide when tested against the two mosquito species. Knockdown responses (37%) were only observed in An. dirus exposed to 1% 0-caryophyllene oxide in the contact trial. beta-Caryophyllene oxide did not show any phototoxic potential (PIF= 0.38) nor was there any significant genotoxic response as indicated by no increase in micro-nucleated cells with or without metabolic activation. beta-Caryophyllene oxide could be considered as a safe repellent, effective against mosquitoes. 2020 text https://www.documentation.ird.fr/hor/fdi:010079579 fdi:010079579 Nararak J., Di Giorgio C., Sukkanon C., Mahiou-Leddet V., Ollivier E., Manguin Sylvie, Chareonviriyaphap T.. Excito-repellency and biological safety of beta-caryophyllene oxide against Aedes albopictus and Anopheles dirus (Diptera : Culicidae). 2020, 210, 105556 [10 p.] EN