Juhel J. B. auteur aut IRD Utama R. S. auteur aut IRD Marques V. auteur aut IRD Vimono I. B. auteur aut IRD Sugeha H. Y. auteur aut IRD Kadarusman auteur aut IRD Pouyaud Laurent auteur aut IRD Dejean T. auteur aut IRD Mouillot D. auteur aut IRD Hocdé Régis auteur aut IRD text journalArticle eng text/pdf born digital access Environmental DNA (eDNA) has the potential to provide more comprehensive biodiversity assessments, particularly for vertebrates in species-rich regions. However, this method requires the completeness of a reference database (i.e. a list of DNA sequences attached to each species), which is not currently achieved for many taxa and ecosystems. As an alternative, a range of operational taxonomic units (OTUs) can be extracted from eDNA metabarcoding. However, the extent to which the diversity of OTUs provided by a limited eDNA sampling effort can predict regional species diversity is unknown. Here, by modelling OTU accumulation curves of eDNA seawater samples across the Coral Triangle, we obtained an asymptote reaching 1531 fish OTUs, while 1611 fish species are recorded in the region. We also accurately predict (R-2 = 0.92) the distribution of species richness among fish families from OTU-based asymptotes. Thus, the multi-model framework of OTU accumulation curves extends the use of eDNA metabarcoding in ecology, biogeography and conservation. specialized eDNA metabarcoding sequence clustering Operational Taxonomic Unit diversity assessment detectability PAPOUASIE NOUVELLE GUINEE INDONESIE 034 020 287 1930 art. 20200248 [10 ] 2020 0962-8452 https://www.documentation.ird.fr/hor/fdi:010079432 10.1098/rspb.2020.0248 0962-8452 [F B010079432] https://www.documentation.ird.fr/hor/fdi:010079432 https://www.documentation.ird.fr/intranet/publi/2020/08/010079432.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2020-09-17 2025-02-24 fdi:010079432 fre