@article{fdi:010079402,
  title = {{C}omparative study of the effects of ziram and disulfiram on human monocyte-derived macrophage functions and polarization : involvement of zinc},
  author = {{P}arny, {M}. and {B}ernad, {J}. and {P}rat, {M}. and {S}alon, {M}. and {A}ubouy, {A}gn{\`e}s and {B}onnafe, {E}. and {C}oste, {A}. and {P}ipy, {B}. and {T}reilhou, {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{Z}iram, a zinc dithiocarbamate is widely used worldwide as a fungicide in agriculture. {I}n order to investigate ziram-induced changes in macrophage functions and polarization, human monocytes-derived macrophages in culture were treated with ziram at 0.01-10 mu mol.{L}(-1)for 4-24 h. {T}o characterize zinc involvement in these changes, we also determined the effects of disulfiram alone (dithiocarbamate without zinc) or in co-incubation with {Z}n{SO}4. {W}e have shown that ziram and disulfiram at 0.01 mu mol.{L}(-1)increased zymosan phagocytosis. {I}n contrast, ziram at 10 mu mol.{L}(-1)completely inhibited this phagocytic process, the oxidative burst triggered by zymosan and the production of {TNF}-alpha, {IL}-1 beta, {IL}-6, and {CCL}2 triggered by {LPS}. {D}isulfiram had the same effects on these macrophages functions only when combined with zinc (10 mu mol.{L}-1). {I}n contrast, at 10 mu mol.{L}(-1)ziram and zinc associated-disulfiram induced expression of several antioxidants genes {HMOX}1, {SOD}2, and catalase, which could suggest the induction of oxidative stress. {T}his oxidative stress could be involved in the increase in late apoptosis induced by ziram (10 mu mol.{L}-1) and zinc associated-disulfiram. {C}oncerning gene expression profiles of membrane markers of macrophage polarization, ziram at 10 mu mol.{L}(-1)had two opposite effects. {I}t inhibited the gene expression of {M}2 markers ({CD}36, {CD}163) in the same way as the disulfiram-zinc co-treatment. {C}onversely, ziram induced gene expression of other {M}2 markers {CD}209, {CD}11b, and {CD}16 in the same way as treatment with zinc alone. {D}isulfiram-zinc association had no significant effects on these markers. {T}hese results taken together show that ziram via zinc modulates macrophages to {M}2-like anti-inflammatory phenotype which is often associated with various diseases.},
  keywords = {{M}acrophages ; {P}olarization ; {P}hagocytosis ; {O}xidative burst ; {C}ytokines ; {P}esticides},
  booktitle = {},
  journal = {{C}ell {B}iology and {T}oxicology},
  volume = {37},
  numero = {3},
  pages = {379--400},
  ISSN = {0742-2091},
  year = {2021},
  DOI = {10.1007/s10565-020-09540-6},
  URL = {https://www.documentation.ird.fr/hor/fdi:010079402},
}