%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Pastorino, B.
%A Touret, F.
%A Gilles, Magali
%A Luciani, L.
%A Lamballerie, X. de
%A Charrel, R. N.
%T Evaluation of chemical protocols for inactivating SARS-CoV-2 infectious samples
%D 2020
%L fdi:010079383
%G ENG
%J Viruses
%K SARS-CoV-2 ; coronavirus ; extraction buffer ; COVID-19 ; inactivation ; lysis buffer
%M ISI:000551106100001
%N 6
%P art. 624 [6 ]
%R 10.3390/v12060624
%U https://www.documentation.ird.fr/hor/fdi:010079383
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers20-08/010079383.pdf
%V 12
%W Horizon (IRD)
%X Clinical samples collected in coronavirus disease 19 (COVID-19), patients are commonly manipulated in biosafety level 2 laboratories for molecular diagnostic purposes. Here, we tested French norm NF-EN-14476+A2 derived from European standard EN-14885 to assess the risk of manipulating infectious viruses prior to RNA extraction. SARS-CoV-2 cell-culture supernatant and nasopharyngeal samples (virus-spiked samples and clinical samples collected in COVID-19 patients) were used to measure the reduction of infectivity after 10 min contact with lysis buffer containing various detergents and chaotropic agents. A total of thirteen protocols were evaluated. Two commercially available formulations showed the ability to reduce infectivity by at least 6 log 10, whereas others proved less effective.
%$ 052 ; 020