@article{fdi:010079383,
  title = {{E}valuation of chemical protocols for inactivating {SARS}-{C}o{V}-2 infectious samples},
  author = {{P}astorino, {B}. and {T}ouret, {F}. and {G}illes, {M}agali and {L}uciani, {L}. and {L}amballerie, {X}. de and {C}harrel, {R}. {N}.},
  editor = {},
  language = {{ENG}},
  abstract = {{C}linical samples collected in coronavirus disease 19 ({COVID}-19), patients are commonly manipulated in biosafety level 2 laboratories for molecular diagnostic purposes. {H}ere, we tested {F}rench norm {NF}-{EN}-14476+{A}2 derived from {E}uropean standard {EN}-14885 to assess the risk of manipulating infectious viruses prior to {RNA} extraction. {SARS}-{C}o{V}-2 cell-culture supernatant and nasopharyngeal samples (virus-spiked samples and clinical samples collected in {COVID}-19 patients) were used to measure the reduction of infectivity after 10 min contact with lysis buffer containing various detergents and chaotropic agents. {A} total of thirteen protocols were evaluated. {T}wo commercially available formulations showed the ability to reduce infectivity by at least 6 log 10, whereas others proved less effective.},
  keywords = {{SARS}-{C}o{V}-2 ; coronavirus ; extraction buffer ; {COVID}-19 ; inactivation ; lysis buffer},
  booktitle = {},
  journal = {{V}iruses},
  volume = {12},
  numero = {6},
  pages = {art. 624 [6 ]},
  year = {2020},
  DOI = {10.3390/v12060624},
  URL = {https://www.documentation.ird.fr/hor/fdi:010079383},
}