@article{fdi:010079366,
  title = {{E}nzymology, histological and ultrastructural effects of ar-turmerone on {C}ulex pipiens pallens larvae},
  author = {{L}iu, {J}. and {F}ernandez, {D}iana and {G}ao, {Y}. {J}. and {S}ilvie, {P}ierre and {G}ao, {Y}. {D}. and {D}ai, {G}. {H}.},
  editor = {},
  language = {{ENG}},
  abstract = {{O}ur previous article demonstrated that ar-turmerone ((6{S})-2-methyl-6-(4-methylphenyl)-2-hepten-4-one) extracted from {C}urcuma longa {L}. has a significant larvicidal activity against the fourth instar larvae of{C}ulex pipiens pallens. {T}o reveal the effects of ar-turmerone on {C}. pipiens pallens larvae, light microscopy and transmission electron microscopy were used to observe the histological and ultrastructure changes in muscle and digestive tissues of fourth instar larvae. {I}t was also revealed by detecting the activity of the acetylcholinesterase ({AC}h{E}) enzyme and three detoxifying enzymes, including carboxylesterase ({C}ar{E}), glutathione-{S}-transferase ({GST}) and {C}ytochrome {P}450 monooxidases ({P}450). {T}he observation under the light microscope showed that the larvae displayed a disruption of myofibril in ventral muscle cells, the disappearance of nucleolus in the malpighian tubule cells, and the exfoliation of the brush border in midgut epithelial cells, 24 h after treatment. {T}he observation under the transmission electron microscope displayed disorganized {Z}-lines in the ventral muscle cells, and dissolved membrane of mitochondria, nuclear and endoplasmic reticulum in abdominal cells. {T}he enzymatic activity results showed that ar-turmerone significantly increased the level of detoxifying enzymes, while the activity of {AC}h{E} was not obviously affected. {A}ll the results suggest that the larvicidal mechanism of ar-turmerone is estimated to be stomach poison and the active sites might be the muscle and digestive tissues, and the mode of action of ar-turmerone may be unrelated to {AC}h{E}.},
  keywords = {phytochemical ; mosquito ; immature stages ; mechanism},
  booktitle = {},
  journal = {{I}nsects},
  volume = {11},
  numero = {6},
  pages = {art. 336 [13 p.]},
  year = {2020},
  DOI = {10.3390/insects11060336},
  URL = {https://www.documentation.ird.fr/hor/fdi:010079366},
}