%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Cheng, S.
%A Pheng, S. H.
%A Seiha, H.
%A Marks, G. B.
%A Banuls, Anne-Laure
%A Mao, T. E.
%A Kerleguer, A.
%T Evaluation of loopamp assay for the diagnosis of pulmonary tuberculosis in Cambodia
%D 2020
%L fdi:010079122
%G ENG
%J Biomed Research International
%@ 2314-6133
%K CAMBODGE
%M ISI:000542664400005
%P 6828043 [7 ]
%R 10.1155/2020/6828043
%U https://www.documentation.ird.fr/hor/fdi:010079122
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers20-07/010079122.pdf
%V 2020
%W Horizon (IRD)
%X The Loopamp (TM) MTBC kit (TB-LAMP) is recommended by WHO for Mycobacterium tuberculosis complex detection in low-income countries with a still low drug-resistant tuberculosis (TB) rate. This study is aimed at testing its feasibility in Cambodia on sputa collected from presumptive tuberculosis patients. 499 samples were tested at a smear microscopy center and 200 at a central-level mycobacteriology laboratory. Using mycobacterial cultures as reference, TB-LAMP results were compared with those of LED fluorescent microscopy (LED-FM) and Xpert (R) MTB/RIF. At the microscopy center, TB-LAMP sensitivity was higher than that of LED-FM (81.5% [95% CI, 74.5-87.6] versus 69.4% [95% CI, 62.2-76.6]), but lower than that of the Xpert assay (95.5% [95% CI 92.3-98.8]). At the central-level laboratory, TB-LAMP sensitivity (92.8% [95% CI, 87.6-97.9]) was comparable to that of Xpert (90.7% [95% CI, 85.0-96.5]) using stored sample. No significant difference in terms of specificity between TB-LAMP and Xpert assays was observed in both study sites. In conclusion, our data demonstrate that TB-LAMP could be implemented at microscopy centers in Cambodia to detect TB patients. In addition, TB-LAMP can be a better choice to replace smear microscopy for rapid TB diagnosis of new presumptive TB patients, in settings with relative low prevalence of drug-resistant TB and difficulties to implement Xpert assay.
%$ 050