@article{fdi:010079007,
  title = {{C}o-acquired nanovirus and geminivirus exhibit a contrasted localization within their common aphid vector},
  author = {{D}i {M}attia, {J}. and {R}yckebusch, {F}. and {V}ernerey, {M}. {S}. and {P}irolles, {E}. and {S}auvion, {N}. and {P}eterschmitt, {M}. and {Z}eddam, {J}ean-{L}ouis and {B}lanc, {S}.},
  editor = {},
  language = {{ENG}},
  abstract = {{S}ingle-stranded {DNA} (ss{DNA}) plant viruses belong to the families {G}eminiviridae and {N}anoviridae. {T}hey are transmitted by {H}emipteran insects in a circulative, mostly non-propagative, manner. {W}hile geminiviruses are transmitted by leafhoppers, treehoppers, whiteflies and aphids, nanoviruses are transmitted exclusively by aphids. {C}irculative transmission involves complex virus-vector interactions in which epithelial cells have to be crossed and defense mechanisms counteracted. {V}ector taxa are considered a relevant taxonomic criterion for virus classification, indicating that viruses can evolve specific interactions with their vectors. {T}hus, we predicted that, although nanoviruses and geminiviruses represent related viral families, they have evolved distinct interactions with their vector. {T}his prediction is also supported by the non-structural {N}uclear {S}huttle {P}rotein ({NSP}) that is involved in vector transmission in nanoviruses but has no similar function in geminiviruses. {T}hanks to the recent discovery of aphid-transmitted geminiviruses, this prediction could be tested for the geminivirus alfalfa leaf curl virus ({ALCV}) and the nanovirus faba bean necrotic stunt virus ({FBNSV}) in their common vector, {A}phis craccivora. {E}stimations of viral load in midgut and head of aphids, precise localization of viral {DNA} in cells of insect vectors and host plants, and virus transmission tests revealed that the pathway of the two viruses across the body of their common vector differs both quantitatively and qualitatively.},
  keywords = {fluorescent in situ hybridization ; q{PCR} ; leaf discs ; {H}emiptera ; nanovirus ; geminivirus ; vector transmission},
  booktitle = {},
  journal = {{V}iruses},
  volume = {12},
  numero = {3},
  pages = {art. 299 [14 p.]},
  year = {2020},
  DOI = {10.3390/v12030299},
  URL = {https://www.documentation.ird.fr/hor/fdi:010079007},
}