@article{fdi:010078967,
  title = {{U}ptake of {P}lasmodium falciparum gametocytes during mosquito bloodmeal by direct and membrane feeding},
  author = {{T}alman, {A}rthur and {O}uologuem, {D}. {T}. {D}. and {L}ove, {K}. and {H}owick, {V}. {M}. and {M}ulamba, {C}. and {H}aidara, {A}. and {D}ara, {N}. and {S}ylla, {D}. and {S}acko, {A}. and {C}oulibaly, {M}. {M}. and {D}ao, {F}. and {S}angare, {C}. {P}. {O}. and {D}jimde, {A}. and {L}awniczak, {M}. {K}. {N}.},
  editor = {},
  language = {{ENG}},
  abstract = {{P}lasmodium falciparum remains one of the leading causes of child mortality, and nearly half of the world's population is at risk of contracting malaria. {W}hile pathogenesis results from replication of asexual forms in human red blood cells, it is the sexually differentiated forms, gametocytes, which are responsible for the spread of the disease. {F}or transmission to succeed, both mature male and female gametocytes must be taken up by a female {A}nopheles mosquito during its blood meal for subsequent differentiation into gametes and mating inside the mosquito gut. {O}bserved circulating numbers of gametocytes in the human host are often surprisingly low. {A} pre-fertilization behavior, such as skin sequestration, has been hypothesized to explain the efficiency of human-to-mosquito transmission but has not been sufficiently tested due to a lack of appropriate tools. {I}n this study, we describe the optimization of a q{PCR} tool that enables the relative quantification of gametocytes within very small input samples. {S}uch a tool allows for the quantification of gametocytes in different compartments of the host and the vector that could potentially unravel mechanisms that enable highly efficient malaria transmission. {W}e demonstrate the use of our gametocyte quantification method in mosquito blood meals from both direct skin feeding on {P}lasmodium gametocyte carriers and standard membrane feeding assay. {R}elative gametocyte abundance was not different between mosquitoes fed through a membrane or directly on the skin suggesting that there is no systematic enrichment of gametocytes picked up in the skin.},
  keywords = {malaria ; transmission ; gametocyte ; mosquito feeding ; {P}lasmodium falciparum},
  booktitle = {},
  journal = {{F}rontiers in {M}icrobiology},
  volume = {11},
  numero = {},
  pages = {art. 246 [7 p.]},
  ISSN = {1664-302{X}},
  year = {2020},
  DOI = {10.3389/fmicb.2020.00246},
  URL = {https://www.documentation.ird.fr/hor/fdi:010078967},
}