Ranoarisoa M. P. auteur aut IRD Trap Jean auteur aut IRD Pablo Anne-Laure auteur aut IRD Dezette D. auteur aut IRD Plassard C. auteur aut IRD text journalArticle eng text/pdf born digital access Phytate is considered a poorly available plant P source but proved to be useful for particular soil bacteria strains. In soil-free conditions, it has been shown that bacteria locked up the mineralized phosphorus from phytate whereas bacterial grazers like nematodes were able to deliver P to plants. Here, we aimed to determine if the interactions between phytate-mineralizing bacteria, bacterial grazer nematodes, and mycorrhizal fungi could increase plant P acquisition from phytate in high P-adsorbing soils. Pinus pinaster was grown in a Cambisol supplemented with phytate. Plants, whether associated or not associated with the ectomycorrhizal fungus Hebeloma cylindrosporum, were either inoculated or not inoculated with the phytase-releasing bacteria Bacillus subtilis and the bacterial-feeding nematode Rhabditis sp. After 100 days, the dual inoculation of bacteria and nematodes significantly increased net plant P accumulation. We observed that, on average, mycorrhizal plants accumulated more P in their shoots than non-mycorrhizal plants. However, the highest plant P acquisition efficiency was found when the three soil organisms were present in the P. pinaster rhizosphere. We conclude that, in a highly inorganic P-fixing soil, plant P acquisition from phytate strongly depends on the grazing of phytate-mineralizing bacteria. Our results confirm the importance of the soil microbial loop to improve plant P nutrition from phytate, which should be considered a route to improve the utilization of this source of poorly available P by plants. specialized Phosphorus cycling Microbial loop Bacillus subtilis Pinus pinaster Hebeloma cylindrosporum Rhabditis sp 068 074 076 143 107728 [10 ] 2020 0038-0717 https://www.documentation.ird.fr/hor/fdi:010078924 10.1016/j.soilbio.2020.107728 0038-0717 [F B010078924] https://www.documentation.ird.fr/hor/fdi:010078924 https://www.documentation.ird.fr/intranet/publi/2020/04/010078924.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2020-05-19 2025-02-24 fdi:010078924 fre