@article{fdi:010078835, title = {{E}valuating the probability of {CRISPR}-based gene drive contaminating another species}, author = {{C}ourtier-{O}rgogozo, {V}. and {D}anchin, {A}. and {G}ouyon, {P}.{H}. and {B}o{\¨e}te, {C}hristophe}, editor = {}, language = {{ENG}}, abstract = {{T}he probability {D} that a given clustered regularly interspaced short palindromic repeats ({CRISPR}) based gene drive element contaminates another, nontarget species can be estimated by the following {D}rive {R}isk {A}ssessment {Q}uantitative {E}stimate ({DRAQUE}) {E}quation: {D} = (hyb + transf) x express x cut x flank x immune x nonextinct with hyb = probability of hybridization between the target species and a nontarget species; transf = probability of horizontal transfer of a piece of {DNA} containing the gene drive cassette from the target species to a nontarget species (with no hybridization); express = probability that the {C}as9 and guide {RNA} genes are expressed; cut = probability that the {CRISPR} guide {RNA} recognizes and cuts at a {DNA} site in the new host; flank = probability that the gene drive cassette inserts at the cut site; immune = probability that the immune system does not reject {C}as9 expressing cells; nonextinct = probability of invasion of the drive within the population. {W}e discuss and estimate each of the seven parameters of the equation, with particular emphasis on possible transfers within insects, and between rodents and humans. {W}e conclude from current data that the probability of a gene drive cassette to contaminate another species is not insignificant. {W}e propose strategies to reduce this risk and call for more work on estimating all the parameters of the formula.}, keywords = {}, booktitle = {}, journal = {{E}volutionary {A}pplications}, volume = {13}, numero = {8}, pages = {1888--1905}, ISSN = {1752-4571}, year = {2020}, DOI = {10.1111/eva.12939}, URL = {https://www.documentation.ird.fr/hor/fdi:010078835}, }