Morlais Isabelle auteur aut IRD Grébaut Pascal auteur aut IRD Bodo J.M. auteur aut IRD Djoba S. auteur aut IRD Cuny Gérard auteur aut IRD Herder Stéphane auteur aut IRD text journalArticle eng text/pdf reformatted digital access The prevalence of various species and subgroups of trypanosomes in infected flies from three sleeping sickness foci in Cameroon was determined by the use of polymerase chain reaction (PCR). The predominant tsetse species found were Glossina palpalis palpalis. Microscopical examination of 943 non-teneral tsetse flies revealed an average infection rate of 10.4%. A total of 90 flies were analyzed for trypanosome identification with primer sets specific for Trypanosoma (Trypanozoon) brucei s.l., T. (Duttonella) vivax, T. (Nannomonas) simiae, and forest type T. (Nannamonas) congolense. PCR succeeded in identifying 52 of the 90 infected flies. Other primers were also tested on microscope positive/PCR-negative infections, and trypanosome subgroups were detected (Kilifi type and savannah type T. congolense). PCR amplification allowed identification of immature infections and revealed mixed-infections. The PCR technique failed to identify 42.2% (38/90) of the parasitologically positive flies and the reasons for this failure are discussed. specialized TRYPANOSOMIASE HUMAINE MALADIE DU SOMMEIL VECTEUR ISOLEMENT D'AGENT PATHOGENE PREVALENCE PCR.REACTION DE POLYMERISATION EN CHAINE CAMEROUN 052GLOTRY 020BIOL 70 1 109-117 1998 0001-706X https://www.documentation.ird.fr/hor/fdi:010078593 10.1016/S0001-706X(98)00014-X 0001-706X [F B010078593] https://www.documentation.ird.fr/hor/fdi:010078593 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers20-07/010078593.pdf IRD - Base Horizon / Pleins textes 2020-07-13 2020-07-31 fdi:010078593 fre