@article{fdi:010078198,
  title = {{B}acterial microbiome in wild-caught {A}nopheles mosquitoes in {W}estern {T}hailand},
  author = {{T}ainchum, {K}. and {D}upont, {C}. and {C}hareonviriyaphap, {T}. and {J}umas-{B}ilak, {E}. and {B}angs, {M}. {J}. and {M}anguin, {S}ylvie},
  editor = {},
  language = {{ENG}},
  abstract = {{A}mong the complex microbial community living in the mosquito midgut, some bacteria (e.g., {E}nterobacter spp.) can deliver effector molecules with anti-{P}lasmodium effects suppressing the development of malaria parasites ({P}lasmodium falciparum) before the ookinete can penetrate the mosquito midgut epithelium. {D}espite knowledge of this phenomenon, only a few studies have defined the diversity of microbiota in wild-caught adult {A}nopheles species. {T}he objective of this study was to analyze and compare the bacterial microbiota in different {A}nopheles species, including representatives of the primary malaria vectors in western {T}hailand. {W}ild female {A}nopheles species were sampled from malaria-endemic areas in {T}ak and {M}ae {H}ong {S}on provinces near the {T}hai-{M}yanmar border. {M}idgut/abdominal bacterial diversity was assessed by examining the 16{S} r{RNA} gene, {V}3 hypervariable region, using {PCR}-{T}emporal {T}emperature {G}el {E}lectrophoresis ({PCR}-{TTGE}) profiling and sequence analysis. {A} total of 24 bacterial genera were identified from eight {A}nopheles species. {F}ive bacterial genera were newly reported in {A}nopheles mosquitoes ({F}errimonas, {M}egasphaera, {P}ectobacterium, {S}himwellia, and {T}rabulsiella). {F}ive genera, including {M}egasphaera, were detected exclusively in a single-malaria ({P}lasmodium vivax) infected {A}nopheles minimus and not observed in other non-infected mosquitoes. {T}he use of {PCR}-{TTGE} provides the first characterization of the midgut bacterial microbiome present in wild adult {A}nopheles in {T}hailand. {E}vidence that microbiota might impact pathogen development (suppression) in {A}nopheles and thereby reduce the risk of pathogen transmission deserves more studies to describe the presence and better understand the biological role of bacteria in natural mosquito populations.},
  keywords = {{A}nopheles mosquitoes ; malaria ; bacterial microbiota ; biodiversity ; {T}hailand ; {THAILANDE}},
  booktitle = {},
  journal = {{F}rontiers in {M}icrobiology},
  volume = {11},
  numero = {},
  pages = {art. 965 [12p.]},
  ISSN = {1664-302{X}},
  year = {2020},
  DOI = {10.3389/fmicb.2020.00965},
  URL = {https://www.documentation.ird.fr/hor/fdi:010078198},
}