@article{fdi:010078185,
  title = {{H}arnessing dengue rapid diagnostic tests for the combined surveillance of dengue, zika, and chikungunya viruses in {L}aos},
  author = {{V}ongsouvath, {M}. and {B}harucha, {T}. and {S}eephonelee, {M}. and {L}amballerie, {X}. de and {N}ewton, {P}. {N}. and {D}ubot {P}{\'e}r{\`e}s, {A}udrey},
  editor = {},
  language = {{ENG}},
  abstract = {{R}ecent expansions of vector-borne diseases highlight the need for improved surveillance, especially in resource-poor settings. {D}engue virus ({DENV}), chikungunya virus ({CHIKV}), and {Z}ika virus ({ZIKV}) share the same vectors as well as similar clinical presentations, suggesting that combined surveillance would be useful. {W}e hypothesized that blood spotted on dengue rapid diagnostic tests ({RDT}s) could be harnessed for sample collection in remote areas for subsequent detection of {DENV}, {CHIKV}, and {ZIKV} by reverse transcription real-time polymerase chain reaction ({RT}-q{PCR}). {CHIKV} and {ZIKV} dilutions were spotted on dengue {RDT}s ({SD} {BIOLINE} {D}engue {DUO}, {S}tandard {D}iagnostics, {G}yeonggi-do, {R}epublic of {K}orea), dried, and extracted. {A}s reference, aliquots of each viral dilution were directly extracted. {U}sing specific {RT}-q{PCR} tests, both viruses were successfully detected from {RDT} extracts. {H}owever, the limit of detection was slightly lower in comparison to direct extracts, two logfold for {CHIKV} and one logfold for {ZIKV}. {F}or analysis of temperature stability, {DENV} dilutions were spotted on {RDT}s and stored for up to 2 months at -80 degrees {C}, 4 degrees {C}, or 35 degrees {C} before testing. {S}torage of {RDT}s for 2 months at 35 degrees {C} did not compromise detection of {RNA} by {RT}-q{PCR}; only minimal degradation was observed. {T}his proof-of-principle study demonstrates the potential of using dengue {RDT}s for {DENV}/{CHIKV}/{ZIKV} combined surveillance in areas without access to laboratory facilities. {F}urther investigations are needed for evaluation of tri-viral surveillance under field conditions using patient samples. {L}arge-scale implementation of surveillance for these viruses is of crucial public health importance for the early detection of epidemics. {T}his method also has important implications for improving understanding of the molecular epidemiology of the three viruses.},
  keywords = {{LAOS}},
  booktitle = {},
  journal = {{A}merican {J}ournal of {T}ropical {M}edicine and {H}ygiene},
  volume = {102},
  numero = {6},
  pages = {1244--1248},
  ISSN = {0002-9637},
  year = {2020},
  DOI = {10.4269/ajtmh.19-0881},
  URL = {https://www.documentation.ird.fr/hor/fdi:010078185},
}