@article{fdi:010078128,
  title = {{E}ffect of irradiation on the survival and susceptibility of female {A}nopheles arabiensis to natural isolates of {P}lasmodium falciparum},
  author = {{G}uissou, {E}. and {P}oda, {S}. and {H}ien, {D}. {F}. {D}. and {Y}erbanga, {S}. {R}. and {D}a, {D}. {F}. and {C}ohuet, {A}nna and {F}ournet, {F}lorence and {R}oux, {O}livier and {M}aiga, {H}. and {D}iabate, {A}. and {G}illes, {J}. and {B}ouyer, {J}. and {O}uedraogo, {A}. {G}. and {R}ayaisse, {J}. {B}. and {L}ef{\`e}vre, {T}hierry and {D}abire, {K}. {R}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground{T}he sterile insect technique ({SIT}) is a vector control strategy relying on the mass release of sterile males into wild vector populations. {C}urrent sex separation techniques are not fully efficient and could lead to the release of a small proportion of females. {I}t is therefore important to evaluate the effect of irradiation on the ability of released females to transmit pathogens. {T}his study aimed to assess the effect of irradiation on the survival and competence of {A}nopheles arabiensis females for {P}lasmodium falciparum in laboratory conditions.{M}ethods{P}upae were irradiated at 95 {G}y of gamma-rays, and emerging females were challenged with one of 14 natural isolates of {P}. falciparum. {S}even days post-blood meal (dpbm), irradiated and unirradiated-control females were dissected to assess the presence of oocysts, using 8 parasite isolates. {O}n 14 dpbm, sporozoite dissemination in the head/thorax was also examined, using 10 parasites isolates including 4 in common with the 7 dpbm dissection (oocyst data). {T}he survivorship of irradiated and unirradiated-control mosquitoes was monitored.{R}esults{O}verall, irradiation reduced the proportion of mosquitoes infected with the oocyst stages by 17% but this effect was highly inconsistent among parasite isolates. {S}econdly, there was no significant effect of irradiation on the number of developing oocysts. {T}hirdly, there was no significant difference in both the sporozoite infection rate and load between the irradiated and unirradiated-control mosquitoes. {F}ourthly, irradiation had varying effects on female survival with either a negative effect or no effect.{C}onclusions{T}he effect of irradiation on mosquito competence strongly varied among parasite isolates. {B}ecause of such isolate variability and, the fact that different parasite isolates were used to collect oocyst and sporozoite data, the irradiation-mediated reduction of oocyst prevalence was not confirmed for the sporozoite stages. {O}ur data indicate that irradiated female {A}n. arabiensis could contribute to malaria transmission, and highlight the need for perfect sexing tools, which would prevent the release of females as part of {SIT} programmes.},
  keywords = {{S}terile insect technique ({SIT}) ; {C}ompetence ; {D}irect membrane feeding ; assay ; {BURKINA} {FASO} ; {BOBO} {DIOULASSO}},
  booktitle = {},
  journal = {{P}arasites and {V}ectors},
  volume = {13},
  numero = {1},
  pages = {art. 266 [11 p.]},
  ISSN = {1756-3305},
  year = {2020},
  DOI = {10.1186/s13071-020-04135-w},
  URL = {https://www.documentation.ird.fr/hor/fdi:010078128},
}