@article{fdi:010078097,
  title = {{CRISPR}-mediated accelerated domestication of {A}frican rice landraces},
  author = {{L}acchini, {E}. and {K}iegle, {E}. and {C}astellani, {M}. and {A}dam, {H}{\'e}l{\`e}ne and {J}ouannic, {S}tefan and {G}regis, {V}. and {K}ater, {M}. {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{A}frican {O}ryza glaberrima and {O}ryza sativa landraces are considered valuable resources for breeding traits due to their adaptation to local environmental and soil conditions. {T}hey often possess superior resistance to endemic pests and tolerance to drought and nutrient deficiencies when compared to the "imported" high production {A}sian rice varieties. {I}n contrast, "domestication traits" such as seed shattering, lodging, and seed yield are not well established in these {A}frican landraces. {T}herefore, the use of these {A}frican varieties for high production agriculture is limited by unpredictable yield and grain quality. {W}e are addressing this shortcoming by developing protocols for genetically transforming {A}frican landraces to allow the use of {CRISPR}-{C}as mediated breeding approaches. {H}ere we use as proof of concept the cultivated {A}frican landrace {K}abre to target selected known "domestication loci" and improve the agronomic potential of {K}abre rice. {S}table genetic transformation with {CRISPR}-{C}as9-based vectors generated single and simultaneous multiple gene knockouts. {P}lants with reduced stature to diminish lodging were generated by disrupting the {HTD}1 gene. {F}urthermore, three loci shown to control seed size and/or yield ({GS}3, {GW}2 and {GN}1{A}) were targeted using a multiplex {CRISPR}-{C}as9 construct. {T}his resulted in mutants with significantly improved seed yield. {O}ur study provides an example of how new breeding technologies can accelerate the development of highly productive {A}frican landrace rice varieties, an important advancement considering that {A}frica is a hotspot for worldwide population growth and therefore prone to food shortage.},
  keywords = {},
  booktitle = {},
  journal = {{PL}o{S} {O}ne},
  volume = {15},
  numero = {3},
  pages = {art. e0229782 [12 p.]},
  ISSN = {1932-6203},
  year = {2020},
  DOI = {10.1371/journal.pone.0229782},
  URL = {https://www.documentation.ird.fr/hor/fdi:010078097},
}