@article{fdi:010078093,
  title = {{G}enotyping {E}chinococcus multilocularis in human alveolar echinococcosis patients : an {E}ms{B} microsatellite analysis},
  author = {{K}napp, {J}. and {G}ottstein, {B}. and {B}retagne, {S}. and {B}art, {J}ean-{M}athieu and {U}mhang, {G}. and {R}ichou, {C}. and {B}resson-{H}adni, {S}. and {M}illon, {L}.},
  editor = {},
  language = {{ENG}},
  abstract = {{F}or clinical epidemiology specialists, connecting the genetic diversity of {E}chinococcus multilocularis to sources of infection or particular sites has become somewhat of a holy grail. {I}t is very difficult to trace the infection history of alveolar echinococcosis ({AE}) patients as there may be an incubation period of five to 15 years before reliable diagnosis. {M}oreover, the variability of parasitic manifestations in human patients raises the possibility of genetically different isolates of {E}. multilocularis having different levels of pathogenicity. {T}hus, the exposure of human patients to different strains or genotypes circulating in geographically different environments may lead to different disease outcomes. {M}olecular tools, such as the microsatellite marker {E}ms{B}, were required to investigate these aspects. {T}his genetic marker was previously tested on a collection of 1211 {E}uropean field samples predominantly of animal origin, referenced on a publicly available database. {I}n this study, we investigated a panel of 66 metacestode samples (between 1981 and 2019) recovered surgically from 63 patients diagnosed with alveolar echinococcosis originating from four {E}uropean countries ({F}rance, {S}witzerland, {G}ermany, {B}elgium). {I}n this study, we identified nine {E}ms{B} profiles, five of which were found in patients located in the same areas of {F}rance and {S}witzerland. {O}ne profile was detected on both sides of the {F}rench-{S}wiss border, whereas most patients from non-endemic regions clustered together in another profile. {E}ms{B} profiles appeared to remain stable over time because similar profiles were detected in patients who underwent surgery recently and patients who underwent surgery some time ago. {T}his study sheds light on possible pathways of contamination in humans, including proximity contamination in some cases, and the dominant contamination profiles in {E}urope, particularly for extrahepatic lesions.},
  keywords = {{A}lveolar echinococcosis ; {E}chinococcus multilocularis ; {E}uropean endemic area ; contamination event ; genotyping ; microsatellite {E}ms{B} ; {EUROPE} ; {FRANCE} ; {SUISSE} ; {ALLEMAGNE} ; {BELGIQUE}},
  booktitle = {},
  journal = {{P}athogens},
  volume = {9},
  numero = {4},
  pages = {art. 282 [21 p.]},
  year = {2020},
  DOI = {10.3390/pathogens9040282},
  URL = {https://www.documentation.ird.fr/hor/fdi:010078093},
}