@article{fdi:010078013,
  title = {{C}ryotolerance of somatic embryos of guinea ({P}etiveria alliacea) to {V}-cryoplate technique and histological analysis of their structural integrity},
  author = {{P}ettinelli, {J}. {D}. and {S}oares, {B}. {D}. and {C}ollin, {M}yriam and {M}ansur, {E}. {A}. and {E}ngelmann, {F}lorent and {G}agliardi, {R}. {F}.},
  editor = {},
  language = {{ENG}},
  abstract = {{O}ptimized cryopreservation protocols are essential for the safe, cost-effective and long-term conservation of germplasm of great economic interest, such as {G}uinea ({P}etiveria alliacea {L}.), a medicinal species that synthesizes a great diversity of bioactive substances, among them polysulfides with antitumor activity. {S}omatic embryos ({SE}s) produced from in vitro roots were cryopreserved using the {V}-cryoplate technique. {T}heir viability was measured using the triphenyltetrazolium test and their recovery by counting the number of secondary {SE}s produced per cryopreserved {SE} 90 days after liquid nitrogen ({LN}) exposure. {S}tructural alterations were evaluated qualitatively and quantitatively during the successive stages of the protocol. {SE}s were dehydrated in 0.5 {M} sucrose, attached to cryoplates using sodium alginate solution (3%) and treated with {PVS}2 for different periods. {A}fter immersion in {LN}, {SE}s were rewarmed in unloading solution (1.2 {M} sucrose) at room temperature (25 degrees {C}) for 20 min. {V}iability based on the triphenyltetrazolium test was 100% after 15 min of treatment with {PVS}2 and {LN} exposure, and recovery, based on multiplication rate, was 21 somatic embryos produced per cryopreserved somatic embryo after 90 days of culture. {T}he histological analysis of cryopreserved somatic embryos showed plasmolysis in the different cell types after treatment with {PVS}2, with meristematic and parenchymatic cells presenting a higher plasmolysis level. {H}owever, after rewarming, the level of plasmolysis decreased over cultivation time, reaching only 2% in meristematic cells after 30 days, indicating the good ability of {P}etiveria alliacea {SE}s to develop cryotolerance under the experimental conditions tested.},
  keywords = {{P}lasmolysis ; {C}ryopreservation ; {PVS}2 ; {C}ryoinjury},
  booktitle = {},
  journal = {{A}cta {P}hysiologiae {P}lantarum},
  volume = {42},
  numero = {3},
  pages = {art. 40 [10 p.]},
  ISSN = {0137-5881},
  year = {2020},
  DOI = {10.1007/s11738-019-3003-x},
  URL = {https://www.documentation.ird.fr/hor/fdi:010078013},
}