@article{fdi:010078011,
  title = {{C}omparative study of promastigote- and amastigote-initiated infection of {L}eishmania infantum ({K}inetoplastida : {T}rypanosomatidae) in {P}hlebotomus perniciosus ({D}iptera : {P}sychodidae) conducted in different biosafety level laboratories},
  author = {{V}aselek, {S}. and {P}rudhomme, {J}. and {M}yskova, {J}. and {L}estinova, {T}. and {S}pitzova, {T}. and {B}anuls, {A}nne-{L}aure and {V}olf, {P}.},
  editor = {},
  language = {{ENG}},
  abstract = {{S}and flies ({D}iptera: {P}sychodidae) are natural vectors of {L}eishmania. {F}or the initiation of sand fly experimental infections either {L}eishmania amastigotes or promastigotes can be used. {I}n order to obtain comparable results, it is necessary to adjust and standardize procedures. {D}uring this study, we conducted promastigote- and amastigote-initiated infections of {L}eishmania infantum {N}icolle, 1908 parasites in {P}hlebotomus ({L}arroussius) perniciosus {N}ewstead, 1911 in two laboratories with different levels of biosafety protection. {P}rotocol originally designed for a biosafety level 2 facility was modified for biosafety level 3 facility and infection parameters were compared. {P}articularly, specially designed plastic containers were used for blood feeding; feeders were placed outside the sand fly cage, on the top of the mesh; feeding was performed inside the climatic chamber; separation of engorged females was done in {P}etri dishes kept on ice; engorged females were kept in the cardboard containers until dissection. {A}ll experiments, conducted in both laboratories, resulted in fully developed late stage infections with high number of parasites and colonization of the stomodeal valve. {W}e demonstrated that protocol originally designed for biosafety level 2 facilities can be successfully modified for other biosafety facilities, depending on the special requirements of the individual institution/laboratory.},
  keywords = {{L}eishmania ; amastigote ; promastigote ; infection ; sand fly},
  booktitle = {},
  journal = {{J}ournal of {M}edical {E}ntomology},
  volume = {57},
  numero = {2},
  pages = {601--607},
  ISSN = {0022-2585},
  year = {2020},
  DOI = {10.1093/jme/tjz199},
  URL = {https://www.documentation.ird.fr/hor/fdi:010078011},
}