@article{fdi:010078003,
  title = {{H}ow deep can ectomycorrhizas go? {A} case study on {P}isolithus down to 4 meters in a {B}razilian eucalypt plantation},
  author = {{R}obin, {A}. and {P}radier, {C}. and {S}anguin, {H}. and {M}ah{\'e}, {F}. and {L}ambais, {G}. {R}. and {P}ereira, {A}. {P}. {D}. and {G}ermon, {A}. and {S}antana, {M}. {C}. and {T}isseyre, {P}ierre and {P}ablo, {A}nne-{L}aure and {H}euillard, {P}. and {S}auvadet, {M}arie and {B}ouillet, {J}. {P}. and {A}ndreote, {F}. {D}. and {P}lassard, {C}. and {G}on{\c{c}}alves, {J}. {L}. {D}. and {C}ardoso, {E}jbn and {L}aclau, {J}. {P}. and {H}insinger, {P}. and {J}ourdan, {C}.},
  editor = {},
  language = {{ENG}},
  abstract = {{D}espite the strong ecological importance of ectomycorrhizal ({ECM}) fungi, their vertical distribution remains poorly understood. {T}o our knowledge, {ECM} structures associated with trees have never been reported in depths below 2 meters. {I}n this study, fine roots and {ECM} root tips were sampled down to 4-m depth during the digging of two independent pits differing by their water availability. {A} meta-barcoding approach based on {I}llumina sequencing of internal transcribed spacers ({ITS}1 and {ITS}2) was carried out on {DNA} extracted from root samples (fine roots and {ECM} root tips separately). {ECM} fungi dominated the rootassociated fungal community, with more than 90% of sequences assigned to the genus {P}isolithus. {T}he morphological and barcoding results demonstrated, for the first time, the presence of {ECM} symbiosis down to 4-m. {T}he molecular diversity of {P}isolithus spp. was strongly dependent on depth, with soil p{H} and soil water content as primary drivers of the {P}isolithus spp. structure. {A}ltogether, our results highlight the importance to consider the {ECM} symbiosis in deep soil layers to improve our understanding of fine roots functioning in tropical soils.},
  keywords = {{D}eep fine roots ; {ECM} root tips ; {E}ucalyptus grandis ; {N}ext-generation sequencing ; {D}iversity ; {T}ropical forest plantations ; {BRESIL} ; {ZONE} {TROPICALE}},
  booktitle = {},
  journal = {{M}ycorrhiza},
  volume = {29},
  numero = {6},
  pages = {637--648},
  ISSN = {0940-6360},
  year = {2019},
  DOI = {10.1007/s00572-019-00917-y},
  URL = {https://www.documentation.ird.fr/hor/fdi:010078003},
}