@article{fdi:010077980, title = {{A}dvances in cryopreservation of in vitro-derived propagules : technologies and explant sources}, author = {{W}ang, {M}. {R}. and {L}ambardi, {M}. and {E}ngelmann, {F}lorent and {P}athirana, {R}. and {P}anis, {B}. and {V}olk, {G}. {M}. and {W}ang, {Q}. {C}.}, editor = {}, language = {{ENG}}, abstract = {{K}ey message {N}ew technologies for in-vitro based cryopreservation systems have advanced the field of plant cryopreservation since the twenty first century. {F}urther advances will certainly facilitate even more diverse germplasm to be successfully preserved in cryobanks. {G}enetic improvements in plant breeding are dependent upon having access to novel plant genetic resources that are available in plant genebanks. {M}any crops that are vegetatively-propagated are maintained as plants in the field or greenhouse, making them vulnerable to biotic and abiotic threats. {I}ncreasingly, plant genebanks are using cryopreservation technologies to secure vegetatively propagated collections at secondary locations. {D}roplet vitrification and cryo-plate cryopreservation methods have been used to successfully cryopreserve the shoot tips of many plant species. {N}ew propagule types, including small leaf square-bearing adventitious buds, stem disc-bearing adventitious buds, microtubers and rhizome buds are alternative explants for use in cryopreservation. {T}his review describes new technologies for in-vitro based cryopreservation systems that have advanced the field of plant cryopreservation. {F}uture advances will allow even more diverse germplasm to be successfully preserved in cryobanks.}, keywords = {{C}ryopreservation ; {C}ryo-plate ; {C}ryo-mesh ; {D}roplet-vitrification ; {M}icrotubers ; {S}hoot tips}, booktitle = {}, journal = {{P}lant {C}ell {T}issue and {O}rgan {C}ulture}, volume = {144}, numero = {1}, pages = {7--20}, ISSN = {0167-6857}, year = {2021}, DOI = {10.1007/s11240-020-01770-0}, URL = {https://www.documentation.ird.fr/hor/fdi:010077980}, }