Cordeiro L. D. auteur aut IRD Collin Myriam auteur aut IRD Callado C. H. auteur aut IRD Simoes-Gurgel C. auteur aut IRD Albarello N. auteur aut IRD Engelmann Florent auteur aut IRD text journalArticle eng text/pdf born digital access Adventitious root cultures of Tarenaya rosea were successfully cryopreserved using the encapsulation-vitrification technique. Histological analysis revealed useful information on the successive steps of cryopreservation. Coupled with complementary histochemical approaches, these studies provided cellular and tissue descriptions of T. rosea root cultures during cryopreservation and contributed to an understanding of cellular stress responses, as well as characterization of the anatomical pattern of root regeneration. The effects of exposure duration to PVS3 solution (0-120 min), unloading treatment (direct and gradual), and recovery medium (liquid and solid) on recovery of cryopreserved roots were investigated. The highest recovery (91%) after cooling in liquid nitrogen (LN) was reached with PVS3 treatment for 90 min, gradual rehydration in unloading solution, and recovery on solid MS medium. The cryopreserved roots showed high multiplication capacity, which was maintained for up to four subcultures. The effect of cryopreservation on root structure was investigated by histological and histochemical studies. Plasmolysis intensified during exposure to loading and PVS3 solutions, but decreased after unloading treatment. The proportion of intercellular spaces increased progressively throughout the cryopreservation protocol, culminating in root cortex disruption. Histochemical analyses revealed polysaccharides, proteins, and both lipidic and pectic substances in intercellular spaces. The vascular cylinder remained intact, ensuring the formation of new roots from the pericycle, showing that proliferative capacity of cryopreserved roots had not diminished. specialized Adventitious root cultures Cleome rosea Intercellular content Medicinal plant Plasmolysis 076 084 257 4 1021-1033 2020 0033-183X https://www.documentation.ird.fr/hor/fdi:010077957 10.1007/s00709-020-01486-0 0033-183X [F B010077957] https://www.documentation.ird.fr/hor/fdi:010077957 https://www.documentation.ird.fr/intranet/publi/2020/03/010077957.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2020-05-18 2022-04-15 fdi:010077957 fre