@article{fdi:010077957,
  title = {{L}ong-term conservation of {T}arenaya rosea ({C}leomaceae) root cultures : histological and histochemical analyses during cryopreservation using the encapsulation-vitrification technique},
  author = {{C}ordeiro, {L}. {D}. and {C}ollin, {M}yriam and {C}allado, {C}. {H}. and {S}imoes-{G}urgel, {C}. and {A}lbarello, {N}. and {E}ngelmann, {F}lorent},
  editor = {},
  language = {{ENG}},
  abstract = {{A}dventitious root cultures of {T}arenaya rosea were successfully cryopreserved using the encapsulation-vitrification technique. {H}istological analysis revealed useful information on the successive steps of cryopreservation. {C}oupled with complementary histochemical approaches, these studies provided cellular and tissue descriptions of {T}. rosea root cultures during cryopreservation and contributed to an understanding of cellular stress responses, as well as characterization of the anatomical pattern of root regeneration. {T}he effects of exposure duration to {PVS}3 solution (0-120 min), unloading treatment (direct and gradual), and recovery medium (liquid and solid) on recovery of cryopreserved roots were investigated. {T}he highest recovery (91%) after cooling in liquid nitrogen ({LN}) was reached with {PVS}3 treatment for 90 min, gradual rehydration in unloading solution, and recovery on solid {MS} medium. {T}he cryopreserved roots showed high multiplication capacity, which was maintained for up to four subcultures. {T}he effect of cryopreservation on root structure was investigated by histological and histochemical studies. {P}lasmolysis intensified during exposure to loading and {PVS}3 solutions, but decreased after unloading treatment. {T}he proportion of intercellular spaces increased progressively throughout the cryopreservation protocol, culminating in root cortex disruption. {H}istochemical analyses revealed polysaccharides, proteins, and both lipidic and pectic substances in intercellular spaces. {T}he vascular cylinder remained intact, ensuring the formation of new roots from the pericycle, showing that proliferative capacity of cryopreserved roots had not diminished.},
  keywords = {{A}dventitious root cultures ; {C}leome rosea ; {I}ntercellular content ; {M}edicinal plant ; {P}lasmolysis},
  booktitle = {},
  journal = {{P}rotoplasma},
  volume = {257},
  numero = {4},
  pages = {1021--1033},
  ISSN = {0033-183{X}},
  year = {2020},
  DOI = {10.1007/s00709-020-01486-0},
  URL = {https://www.documentation.ird.fr/hor/fdi:010077957},
}