@article{fdi:010077953,
  title = {{A}n efficient {RNA}-seq-based segregation analysis identifies the sex chromosomes of {C}annabis sativa},
  author = {{P}rentout, {D}. and {R}azumova, {O}. and {R}hon{\'e}, {B}{\'e}n{\'e}dicte and {B}adouin, {H}. and {H}enri, {H}. and {F}eng, {C}. and {K}afer, {J}. and {K}arlov, {G}. and {M}arais, {G}. {A}. {B}.},
  editor = {},
  language = {{ENG}},
  abstract = {{C}annabis sativa-derived tetrahydrocannabinol ({THC}) production is increasing very fast worldwide. {C}. sativa is a dioecious plant with {XY} {C}hromosomes, and only females ({XX}) are useful for {THC} production. {I}dentifying the sex chromosome sequence would improve early sexing and better management of this crop; however, the {C}. sativa genome projects have failed to do so. {M}oreover, as dioecy in the {C}annabaceae family is ancestral, {C}. sativa sex chromosomes are potentially old and thus very interesting to study, as little is known about old plant sex chromosomes. {H}ere, we {RNA}-sequenced a {C}. sativa family (two parents and 10 male and female offspring, 576 million reads) and performed a segregation analysis for all {C}. sativa genes using the probabilistic method {SEX}-{DET}ector. {W}e identified >500 sex-linked genes. {M}apping of these sex-linked genes to a {C}. sativa genome assembly identified the largest chromosome pair being the sex chromosomes. {W}e found that the {X}-specific region (not recombining between {X} and {Y}) is large compared to other plant systems. {F}urther analysis of the sex-linked genes revealed that {C}. sativa has a strongly degenerated {Y} {C}hromosome and may represent the oldest plant sex chromosome system documented so far. {O}ur study revealed that old plant sex chromosomes can have large, highly divergent nonrecombining regions, yet still be roughly homomorphic.},
  keywords = {},
  booktitle = {},
  journal = {{G}enome {R}esearch},
  volume = {30},
  numero = {2},
  pages = {164--172},
  ISSN = {1088-9051},
  year = {2020},
  DOI = {10.1101/gr.251207.119},
  URL = {https://www.documentation.ird.fr/hor/fdi:010077953},
}