@article{fdi:010077948,
  title = {{S}pecies delimitation in the genus {G}reenwayodendron based on morphological and genetic markers reveals new species},
  author = {{L}issambou, {B}. {J}. and {C}ouvreur, {T}homas and {A}tteke, {C}. and {S}t{\'e}vart, {T}. and {P}ineiro, {R}. and {D}auby, {G}illes and {M}onthe, {F}. {K}. and {I}kabanga, {D}. {U}. and {S}onk{\'e}, {B}. and {M}'{B}atchi, {B}. and {H}ardy, {O}. {J}.},
  editor = {},
  language = {{ENG}},
  abstract = {{C}ombining genetic and morphological markers is a powerful approach for species delimitation, much needed in tropical species complexes. {G}reenwayodendron ({A}nnonaceae) is a widespread genus of trees distributed from {W}est to {E}ast {A}frican rainforests. {T}wo species and four infra-specific taxa are currently recognized. {H}owever, preliminary genetic studies and morphological observations suggested the occurrence of additional species, undescribed to date. {W}e tested species delimitation within {G}reenwayodendron by combining morphological and population genetics data. {F}irst, a visual inspection of about a thousand specimens suggested the existence of seven morphogroups: four of them occur in {C}entral {A}frica and overlap in {G}abon while three others are allopatric, occurring respectively in {W}est {A}frica, {E}ast {A}frica, and the islands of {S}ao {T}ome and {P}rincipe. {T}heir morphological differentiation was confirmed by analysis of 27 morphological characters coded from 233 herbarium specimens. {S}econd, after genotyping 800 samples at eight nuclear microsatellites, {B}ayesian clustering analyses ({STRUCTURE}) identified four genetic clusters corresponding to the well-sampled morphogroups but failed to separate the three remaining morphogroups represented by few samples. {H}owever, we show that this is an inherent limit of the {STRUCTURE} algorithm, whereas factorial correspondence analysis ({FCA}) and pairwise {F}-{ST} and {R}-{ST} measures confirmed the genetic differentiation of all morphogroups. {W}e considered that a clear genetic differentiation occurring between sympatric populations advocates for recognizing distinct species following the biological species concept. {O}ur analyses highlight that the current taxonomic treatment of {G}reenwayodendron underestimates the total number of species. {W}e identified two new species and support separation at the rank of species of two varieties ({G}. suaveolens subsp. suaveolens var. gabonica, {G}. suaveolens subsp. suaveolens var. suaveolens) and one subspecies ({G}. suaveolens subsp. usambaricum). {T}he taxonomic status of specimens collected in {S}ao {T}ome and {P}rincipe remains inconclusive, partly due to the limited fertile material available. {O}ur study highlights the strength of combining morphological and population genetics data for discovering new taxa. {G}uidelines for using genetic clustering approaches in species delimitation are provided.},
  keywords = {{A}frica ; {A}nnonaceae ; cryptic species ; genetic clustering ; {G}reenwayodendron ; {AFRIQUE} ; {ZONE} {EQUATORIALE}},
  booktitle = {},
  journal = {{T}axon},
  volume = {68},
  numero = {3},
  pages = {442--454},
  ISSN = {0040-0262},
  year = {2019},
  DOI = {10.1002/tax.12064},
  URL = {https://www.documentation.ird.fr/hor/fdi:010077948},
}