@article{fdi:010077881,
  title = {{I}n situ metabolic activities of uncultivated {F}errovum sp. {CARN}8 evidenced by metatranscriptomic analysis},
  author = {{P}lewniak, {F}. and {K}oechler, {S}. and {L}e {P}aslier, {D}. and {H}ery, {M}. and {B}runeel, {O}dile and {B}ertin, {P}. {N}.},
  editor = {},
  language = {{ENG}},
  abstract = {{A}mongst iron-oxidizing bacteria playing a key role in the natural attenuation of arsenic in acid mine drainages ({AMD}s), members of the {F}errovum genus were identified in mine effluent or water treatment plants, and were shown to dominate biogenic precipitates in field pilot experiments. {I}n order to address the question of the in situ activity of the uncultivated {F}errovum sp. {CARN}8 strain in the {C}arnoules {AMD}, we assembled its genome using metagenomic and metatranscriptomic sequences and we determined standardized expression values for protein-encoding genes. {O}ur results showed that this microorganism was indeed metabolically active and allowed us to sketch out its metabolic activity in its natural environment. {E}xpression of genes related to the respiratory chain and carbon fixation suggests aerobic energy production coupled to ferrous iron oxidation and chemolithoautotrophic growth. {N}otwithstanding the presence of nitrogenase genes in its genome, expression data also indicated that {F}errovum sp. {CARN}8 relied on ammonium import rather than nitrogen fixation. {T}he expression of flagellum and chemotaxis genes hints that at least a proportion of this strain population was motile. {F}inally, apart from some genes related to metal resistance showing surprisingly low expression values, genes involved in stress response were well expressed as expected in {AMD}s.},
  keywords = {{G}ene expression ; {I}n situ metabolism ; {M}etagenome assembled genome},
  booktitle = {},
  journal = {{R}esearch in {M}icrobiology},
  volume = {171},
  numero = {1},
  pages = {37--43},
  ISSN = {0923-2508},
  year = {2020},
  DOI = {10.1016/j.resmic.2019.09.008},
  URL = {https://www.documentation.ird.fr/hor/fdi:010077881},
}