@article{fdi:010077875,
  title = {{A} 40-month follow-up of {E}bola virus disease survivors in {G}uinea ({P}ost{E}bogui) reveals long-term detection of {E}bola viral ribonucleic acid in semen and breast milk},
  author = {{K}eita, {A}.{K}. and {V}idal, {N}icole and {T}oure, {A}. and {D}iallo, {M}. {S}. {K}. and {M}agassouba, {N}. and {B}aize, {S}. and {M}ateo, {M}. and {R}aoul, {H}. and {M}ely, {S}. and {S}ubtil, {F}. and {K}pamou, {C}. and {K}oivogui, {L}. and {T}raore, {F}. and {S}ow, {M}. {S}. and {A}youba, {A}hidjo and {E}tard, {J}ean-{F}ran{\c{c}}ois and {D}elaporte, {E}. and {P}eeters, {M}artine and {D}esclaux, {A}lice and {G}ranouillac, {B}runo and {I}zard, {S}uzanne and {M}arch, {L}aura and {M}sellati, {P}hilippe and {T}averne, {B}ernard and {P}ost{E}bogui {S}tudy {G}roup},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground. {W}ith the increasing frequency and impact of {E}bola virus disease ({EVD}) outbreaks illustrated by recent epidemics, a good understanding of the extent of viral persistance or ribonucleic acid ({RNA}) detection in body fluids from survivors is urgently needed. {M}ethods. {E}bola viral {RNA} shedding was studied with molecular assays in semen (n = 1368), urine (n = 1875), cervicovaginal fluid (n = 549), saliva (n = 900), breast milk (n = 168), and feces (n = 558) from {EVD} survivors in {G}uinea ({P}ost{E}bogui cohort, n = 802) at a regular base period until 40 months after inclusion. {R}esults. {T}wenty-seven of 277 (9.8%) male survivors tested positive for {E}bola {RNA} in at least 1 semen sample. {T}he probability of remaining positive for {E}bola {RNA} in semen was estimated at 93.02% and 60.12% after 3 and 6 months. {V}iral {RNA} in semen was more frequent in patients with eye pain ({P} = .036), joint pain ({P} = .047), and higher antibody levels to {E}bola virus antigens (nucleoprotein [{P} = .001], glycoprotein [{P} = .05], and viral protein-40 [{P} = .05]). {E}bola {RNA} was only rarely detected in the following body fluids from {EVD} survivors: saliva (1 of 454), urine (2 of 593), breast milk (2 of 168), cervicovaginal secretions (0 of 273), and feces (0 of 330). {R}ibonucleic acid was detected in breast milk 1 month after delivery but 500 days after discharge of {E}bola treatment unit ({ETU}) in 1 woman who became pregnant 7 months after discharge from the {ETU}. {C}onclusions. {T}he frequency and potential long-term presence of viral {RNA} in semen confirmed that systematic prevention measures in male survivors are required. {O}ur observation in breast milk suggests that our knowledge on viral reservoir in immune-privileged sites and its impact are still incomplete.},
  keywords = {body fluids ; breast milk ; {E}bola ; {G}uinea ; semen ; {GUINEE}},
  booktitle = {},
  journal = {{O}pen {F}orum {I}nfectious {D}iseases},
  volume = {6},
  numero = {12},
  pages = {ofz482 [8 ]},
  ISSN = {2328-8957},
  year = {2019},
  DOI = {10.1093/ofid/ofz482},
  URL = {https://www.documentation.ird.fr/hor/fdi:010077875},
}