Next-generation sequencing on insectivorous bat guano : an accurate tool to identify arthropod viruses of potential agricultural concern Bourgarel M. auteur aut IRD Noel V. auteur aut IRD Pfukenyi D. auteur aut IRD Michaux J. auteur aut IRD Andre A. auteur aut IRD Becquart Pierre auteur aut IRD Cerqueira F. auteur aut IRD Barrachina C. auteur aut IRD Boue V. auteur aut IRD Talignani L. auteur aut IRD Matope G. auteur aut IRD Missé Dorothée auteur aut IRD Morand S. auteur aut IRD Liégeois Florian auteur aut IRD text journalArticle eng text/pdf reformatted digital access Viruses belonging to the Dicistroviridae family have attracted a great deal of attention from scientists owing to their negative impact on agricultural economics, as well as their recent identification as potential aetiological agents of febrile illness in human patients. On the other hand, some Dicistroviruses are also studied for their potential biopesticide properties. To date, Dicistrovirus characterized in African mainland remain scarce. By using High-Throughput Sequencing technology on insectivorous bat faeces (Hipposideros Caffer) sampled in a cave used by humans to collect bat guano (bat manure) as fertilizer in Zimbabwe, we characterized the full-length sequences of three Dicistrovirus belonging to the Cripavirus and Aparavirus genus: Big Sioux River Virus-Like (BSRV-Like), Acute Bee Paralysis Virus (ABPV), and Aphid Lethal Paralysis Virus (ALPV). Phylogenetic analyses of ORF-1 and ORF-2 genes showed a complex evolutionary history between BSRV and close viruses, as well as for the Aparavirus genus. Herewith, we provide the first evidence of the presence of Dicistrovirus in Zimbabwe and highlight the need to further document the impact of such viruses on crops, as well as in beekeeping activities in Zimbabwe which represent a crucial source of income for Zimbabwean people. specialized Dicistrovirus Bats Faeces Phylogeny HTS Zimbabwe ZIMBABWE 080 052 020 Viruses 11 12 art. 1102 [14 p.] 2019 https://www.documentation.ird.fr/hor/fdi:010077835 10.3390/v11121102 [F B010077835] https://www.documentation.ird.fr/hor/fdi:010077835 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers20-02/010077835.pdf IRD - Base Horizon / Pleins textes 2020-03-11 2025-02-24 fdi:010077835 fre