@article{fdi:010077778,
  title = {{T}erminalia albida treatment improves survival in experimental cerebral malaria through reactive oxygen species scavenging and anti-inflammatory properties},
  author = {{C}amara, {A}. and {H}addad, {M}ohamed and {R}eybier, {K}. and {T}raore, {M}. {S}. and {B}alde, {M}. {A}. and {R}oyo, {J}ade and {B}alde, {A}. {O}. and {B}atigne, {P}. and {H}aidara, {M}. and {B}alde, {E}. {S}. and {C}oste, {A}. and {B}alde, {A}. {M}. and {A}ubouy, {A}gn{\`e}s},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {T}he development of {P}lasmodium resistance to the last effective anti-malarial drugs necessitates the urgent development of new anti-malarial therapeutic strategies. {T}o this end, plants are an important source of new molecules. {T}he objective of this study was to evaluate the anti-malarial effects of {T}erminalia albida, a plant used in {G}uinean traditional medicine, as well as its anti-inflammatory and antioxidant properties, which may be useful in treating cases of severe malaria. {M}ethods: {I}n vitro antiplasmodial activity was evaluated on a chloroquine-resistant strain of {P}lasmodium falciparum ({K}-1). {I}n vivo efficacy of the plant extract was measured in the experimental cerebral malaria model based on {P}lasmodium berghei (strain {ANKA}) infection. {M}ice brains were harvested on {D}ay 7-8 post-infection, and {T} cells recruitment to the brain, expression levels of pro- and anti-inflammatory markers were measured by flow cytometry, {RT}-q{PCR} and {ELISA}. {N}on-malarial in vitro models of inflammation and oxidative response were used to confirm {T}erminalia albida effects. {C}onstituents of {T}erminalia albida extract were characterized by ultra-high performance liquid chromatography coupled with high resolution mass spectrometry. {T}op ranked compounds were putatively identified using plant databases and in silico fragmentation patterns. {R}esults: {I}n vitro antiplasmodial activity of {T}erminalia albida was confirmed with an {IC}50 of 1.5 mu g/m{L}. {I}n vivo, {T}erminalia albida treatment greatly increased survival rates in {P}. berghei-infected mice. {T}reated mice were all alive until {D}ay 12, and the survival rate was 50% on {D}ay 20. {T}erminalia albida treatment also significantly decreased parasitaemia by 100% on {D}ay 4 and 89% on {D}ay 7 post-infection. {I}n vivo anti-malarial activity was related to anti-inflammatory properties, as {T}erminalia albida treatment decreased {T} lymphocyte recruitment and expression of pro-inflammatory markers in brains of treated mice. {T}hese properties were confirmed in vitro in the non-malarial model. {I}n vitro, {T}erminalia albida also demonstrated a remarkable dose-dependent neutralization activity of reactive oxygen species. {T}welve compounds were putatively identified in {T}erminalia albida stem bark. {A}mong them, several molecules already identified may be responsible for the different biological activities observed, especially tannins and triterpenoids. {C}onclusion: {T}he traditional use of {T}erminalia albida in the treatment of malaria was validated through the combination of in vitro and in vivo studies.},
  keywords = {{T}erminalia albida ; {M}alaria ; {E}xperimental cerebral malaria ; {I}nflammatory response ; {O}xidative stress ; {UHPLC}-{HRMS} ; {GUINEE}},
  booktitle = {},
  journal = {{M}alaria {J}ournal},
  volume = {18},
  numero = {1},
  pages = {431 [15 ]},
  year = {2019},
  DOI = {10.1186/s12936-019-3071-9},
  URL = {https://www.documentation.ird.fr/hor/fdi:010077778},
}