@article{fdi:010077751,
  title = {{P}rospecting peptides isolated from black soldier fly ({D}iptera : {S}tratiomyidae) with antimicrobial activity against {H}elicobacter pylori ({C}ampylobacterales : {H}elicobacteraceae)},
  author = {{A}lvarez, {D}. and {W}ilkinson, {K}. {A}. and {T}reilhou, {M}. and {T}ene, {N}. and {C}astillo, {D}. and {S}auvain, {M}ichel},
  editor = {},
  language = {{ENG}},
  abstract = {{H}elicobacter pylori ({M}arshall & {G}oodwin) is a widespread human pathogen that is acquiring resistance to the antibiotics used to treat it.{T}his increasing resistance necessitates a continued search for new antibiotics. {A}n antibiotic source that shows promise is animals whose immune systems must adapt to living in bacteria-laden conditions by producing antibacterial peptides or small molecules. {A}mong these animals is the black soldier fly ({BSF}; {H}ermetia illucens {L}innaeus), a {D}iptera that colonizes decomposing organic matter. {I}n order to find anti-{H}. pylori peptides in {BSF}, larvae were challenged with {E}scherichia coli ({E}nterobacteriales: {E}nterobacteriaceae). {S}mall peptides were extracted from hemolymph and purified using solid-phase extraction, molecular weight cutoff filtration and two rounds of preparative high performance liquid chromatography ({HPLC}). {T}he anti-{H}. pylori fraction was followed through the purification process using the inhibition zone assay in brain-heart infusion agar, while peptides from uninoculated larvae had no activity. {T}he inhibition halo of the active sample was comparable to the action of metronidazole in the inhibition zone assay. {T}he purified sample contained four peptides with average masses of approximately 4.2 k{D}a that eluted together when analyzed by {HPLC}-mass spectrometry. {T}he peptides likely have similar sequences, activity, and properties. {T}herefore, {BSF} produces inducible antibacterial peptides that have in vitro activity against {H}. pylori, which highlights {BSF}'s position as an important target for further bioprospecting.},
  keywords = {{H}ermetia illucens ; bioprospecting ; {H}elicobacter pylori ; antimicrobial ; peptides},
  booktitle = {},
  journal = {{J}ournal of {I}nsect {S}cience},
  volume = {19},
  numero = {6},
  pages = {art. 17 [5 p.]},
  year = {2019},
  DOI = {10.1093/jisesa/iez120},
  URL = {https://www.documentation.ird.fr/hor/fdi:010077751},
}