Ardizzoni E. auteur aut IRD Orikiriza P. auteur aut IRD Ssuuna C. auteur aut IRD Nyehangane D. auteur aut IRD Gumsboga M. auteur aut IRD Taremwa I. M. auteur aut IRD Turyashemererwa E. auteur aut IRD Mwanga-Amumpaire J. auteur aut IRD Langendorf C. auteur aut IRD Bonnet Maryline auteur aut IRD text journalArticle eng text/pdf born digital access Xpert MTB/RIF (Xpert) and culture are the most reliable methods for tuberculosis diagnosis but are still poorly accessible in many low-resource countries. We aimed to assess the effects of OMNIgene Sputum (OM-S) and ethanol in preserving sputum for Xpert and OM-S for mycobacterial growth indicator tube (MGIT) testing over periods of 15 and 8 days, respectively. Sputum samples were collected from newly diagnosed smear-positive patients. For Xpert, pooled samples were split into 5 aliquots: 3 for Xpert on days 0, 7, and 15 without additive and 2 with either OM-S or ethanol at day 15. For MGIT, 2 aliquots were tested without preservative and 2 with OM-S at 0 and 8 days. Totals of 48 and 47 samples were included in the analysis for Xpert and culture. With Xpert, using day 0 as a reference, untreated samples stored for 7 and 15 days showed concordances of 45/46 (97.8%) and 46/48 (95.8%). For samples preserved with OM-S or ethanol for 15 days compared with untreated samples processed at day 0 or after 15 days, OM-S concordances were 46/48 (95.8%) and 47/48 (97.9%), while those of ethanol were 44/48 (91.7%) and 45/48 (93.8%). With MGIT, concordances between untreated and OM-S-treated samples were 21/41 (51.2%) at day 0 and 21/44 (47.7%) at day 8. In conclusion, Xpert equally detected tuberculosis in OM-S-treated and untreated samples up to 15 days but showed slightly lower detection in ethanol-treated samples. Among OM-S-treated samples, MGIT positivity was significantly lower than in untreated samples at both time points. specialized OMNIgene tuberculosis Xpert culture OUGANDA 052 050 084 58 1 e00810-19 [11 ] 2020 0095-1137 https://www.documentation.ird.fr/hor/fdi:010077750 10.1128/jcm.00810-19 0095-1137 [F B010077750] https://www.documentation.ird.fr/hor/fdi:010077750 https://www.documentation.ird.fr/intranet/publi/2020/01/010077750.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2020-02-05 2025-02-24 fdi:010077750 fre