%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Ardizzoni, E.
%A Orikiriza, P.
%A Ssuuna, C.
%A Nyehangane, D.
%A Gumsboga, M.
%A Taremwa, I. M.
%A Turyashemererwa, E.
%A Mwanga-Amumpaire, J.
%A Langendorf, C.
%A Bonnet, Maryline
%T Evaluation of OMNIgene sputum and ethanol reagent for preservation of sputum prior to xpert and culture testing in Uganda
%D 2020
%L fdi:010077750
%G ENG
%J Journal of Clinical Microbiology
%@ 0095-1137
%K OMNIgene ; tuberculosis ; Xpert ; culture
%K OUGANDA
%M ISI:000505473500004
%N 1
%P e00810-19 [11 ]
%R 10.1128/jcm.00810-19
%U https://www.documentation.ird.fr/hor/fdi:010077750
%> https://www.documentation.ird.fr/intranet/publi/2020/01/010077750.pdf
%V 58
%W Horizon (IRD)
%X Xpert MTB/RIF (Xpert) and culture are the most reliable methods for tuberculosis diagnosis but are still poorly accessible in many low-resource countries. We aimed to assess the effects of OMNIgene Sputum (OM-S) and ethanol in preserving sputum for Xpert and OM-S for mycobacterial growth indicator tube (MGIT) testing over periods of 15 and 8 days, respectively. Sputum samples were collected from newly diagnosed smear-positive patients. For Xpert, pooled samples were split into 5 aliquots: 3 for Xpert on days 0, 7, and 15 without additive and 2 with either OM-S or ethanol at day 15. For MGIT, 2 aliquots were tested without preservative and 2 with OM-S at 0 and 8 days. Totals of 48 and 47 samples were included in the analysis for Xpert and culture. With Xpert, using day 0 as a reference, untreated samples stored for 7 and 15 days showed concordances of 45/46 (97.8%) and 46/48 (95.8%). For samples preserved with OM-S or ethanol for 15 days compared with untreated samples processed at day 0 or after 15 days, OM-S concordances were 46/48 (95.8%) and 47/48 (97.9%), while those of ethanol were 44/48 (91.7%) and 45/48 (93.8%). With MGIT, concordances between untreated and OM-S-treated samples were 21/41 (51.2%) at day 0 and 21/44 (47.7%) at day 8. In conclusion, Xpert equally detected tuberculosis in OM-S-treated and untreated samples up to 15 days but showed slightly lower detection in ethanol-treated samples. Among OM-S-treated samples, MGIT positivity was significantly lower than in untreated samples at both time points.
%$ 052 ; 050 ; 084