@article{fdi:010077750,
  title = {{E}valuation of {OMNI}gene sputum and ethanol reagent for preservation of sputum prior to xpert and culture testing in {U}ganda},
  author = {{A}rdizzoni, {E}. and {O}rikiriza, {P}. and {S}suuna, {C}. and {N}yehangane, {D}. and {G}umsboga, {M}. and {T}aremwa, {I}. {M}. and {T}uryashemererwa, {E}. and {M}wanga-{A}mumpaire, {J}. and {L}angendorf, {C}. and {B}onnet, {M}aryline},
  editor = {},
  language = {{ENG}},
  abstract = {{X}pert {MTB}/{RIF} ({X}pert) and culture are the most reliable methods for tuberculosis diagnosis but are still poorly accessible in many low-resource countries. {W}e aimed to assess the effects of {OMNI}gene {S}putum ({OM}-{S}) and ethanol in preserving sputum for {X}pert and {OM}-{S} for mycobacterial growth indicator tube ({MGIT}) testing over periods of 15 and 8 days, respectively. {S}putum samples were collected from newly diagnosed smear-positive patients. {F}or {X}pert, pooled samples were split into 5 aliquots: 3 for {X}pert on days 0, 7, and 15 without additive and 2 with either {OM}-{S} or ethanol at day 15. {F}or {MGIT}, 2 aliquots were tested without preservative and 2 with {OM}-{S} at 0 and 8 days. {T}otals of 48 and 47 samples were included in the analysis for {X}pert and culture. {W}ith {X}pert, using day 0 as a reference, untreated samples stored for 7 and 15 days showed concordances of 45/46 (97.8%) and 46/48 (95.8%). {F}or samples preserved with {OM}-{S} or ethanol for 15 days compared with untreated samples processed at day 0 or after 15 days, {OM}-{S} concordances were 46/48 (95.8%) and 47/48 (97.9%), while those of ethanol were 44/48 (91.7%) and 45/48 (93.8%). {W}ith {MGIT}, concordances between untreated and {OM}-{S}-treated samples were 21/41 (51.2%) at day 0 and 21/44 (47.7%) at day 8. {I}n conclusion, {X}pert equally detected tuberculosis in {OM}-{S}-treated and untreated samples up to 15 days but showed slightly lower detection in ethanol-treated samples. {A}mong {OM}-{S}-treated samples, {MGIT} positivity was significantly lower than in untreated samples at both time points.},
  keywords = {{OMNI}gene ; tuberculosis ; {X}pert ; culture ; {OUGANDA}},
  booktitle = {},
  journal = {{J}ournal of {C}linical {M}icrobiology},
  volume = {58},
  numero = {1},
  pages = {e00810--19 [11 ]},
  ISSN = {0095-1137},
  year = {2020},
  DOI = {10.1128/jcm.00810-19},
  URL = {https://www.documentation.ird.fr/hor/fdi:010077750},
}