@article{fdi:010077736, title = {{E}nhanced hydrogen production by a sequential dark and photo fermentation process : effects of initial feedstock composition, dilution and microbial population}, author = {{N}ino-{N}avarro, {C}. and {C}hairez, {I}. and {C}hristen, {P}ierre and {C}anul-{C}han, {M}. and {G}arcia-{P}ena, {E}. {I}.}, editor = {}, language = {{ENG}}, abstract = {{T}wo-stage process of dark fermentation ({DF}) and photo fermentation ({PF}), using fruit and vegetable waste ({FVW}) and cheese whey powder ({CWP}), was used as an approach to enhance the hydrogen ({H}-2) production. {FVW} and {CWP} at {C}/{N} ratios of 34, 39, 60, 71 and 82 were tested as substrates for {DF}. {D}ilution (1:2, 1:5, 1:10) of the {DF} effluents was used as a coupling strategy. {DF} effluents with low-butyrate and high lactate concentrations were obtained as a function of an increased {C}/{N} ratio, which results in high {H}-2 production during the {PF}. {M}aximum overall {H}-2 yields of 793.7 and 695.4 m{LH}(2)/g{C}hemical {O}xygen {D}emand ({COD}) were obtained using a 1:10 dilution, at a {C}/{N} ratio of 60 and 70, respectively. {T}hese {H}-2 yields were higher than those obtained with the individual processes. {T}he {C}/{N} ratio at the {DF} stage regulate not only {H}-2 production but also the distribution and concentrations of by-products. {T}hese metabolites, in turn, control the {H}-2 production during the {PF}. {P}redominant microbial population for both processes ({DF}: {C}/{N} = 34 {A}cetobacter lovaniensis, {C}lostridium butyricum; {C}/{N} = 39 {C} butyricum, {E}nterobacter sp, {B}ifidobacterium; {C}/{N} = 82 {L}actobacillus casei; {PF}: {R}hodopseudomonas palustris) were in accordance with the final metabolic products.}, keywords = {{T}wo-stage fermentation ; {H}ydrogen production ; {F}ruit and vegetable wastes ; {C}heese whey powder ; {D}ark fermentation ; {P}hoto fermentation}, booktitle = {}, journal = {{R}enewable {E}nergy}, volume = {147}, numero = {1}, pages = {924--936}, ISSN = {0960-1481}, year = {2020}, DOI = {10.1016/j.renene.2019.09.024}, URL = {https://www.documentation.ird.fr/hor/fdi:010077736}, }