@article{fdi:010077730,
  title = {{M}onitoring the presence of trypanosomes' {DNA} - {I}ncluding {T}rypanosoma brucei gambiense {DNA} - {F}rom the midguts of riverine {G}lossina trapped in the south east outskirts of {K}inshasa {C}ity ({D}emocratic {R}epublic of {C}ongo)},
  author = {{G}r{\'e}baut, {P}ascal and {A}ndjingbopou, {Y}. and {M}ansinsa, {D}. {P}. and {M}anzambi, {E}. {Z}. and {M}pembele, {F}. and {L}ejon, {V}eerle and {G}eiger, {A}nne},
  editor = {},
  language = {{ENG}},
  abstract = {{E}ven if the number of {H}uman {A}frican {T}rypanosomiasis ({HAT}) cases from {K}inshasa province in {DRC} is going towards elimination for the last decade, cases still occur in the periphery of the city. {T}he diagnosis of 21 cases in the south periphery of {K}inshasa, between 2015 and 2017 gives evidence of the existence of an active focus in this area. {H}ere, we present the results of a punctual entomological survey that was realized in july 2014 in the outskirts of the southeast of {K}inshasa. {U}sing pyramidal traps, we caught tsetse flies during 2 days, dissecting the fresh ones for further molecular analysis. {T}he average {A}pparent {D}ensity of flies per {T}rap and per {D}ay was three with a maximum of 5.6 flies in {N}ganda {PIO}. {P}olymerase chain reaction analysis of the midguts provided evidence of a high prevalence (57.2%) of infected flies. {N}inety three percent of the trypanosomes that were identified belonged to the {N}anomonas species, but {T}rypanozoon trypanosomes were also present in 24% of the infected flies, including mixed infections with {N}anomonas, including 3 flies carrying {T}rypanosoma brucei gambiense, the human pathogen of trypanosomiasis. {T}hese results show that at the time of the field's study there was an active reservoir of trypanosomes, closed to pigsties, knowing that pig is a potential animal reservoir. {I}t also demonstrates that xenomonitoring using the entomological approach can be an efficient tool for monitoring sleeping sickness. {F}inally, results are discussed in the frame of {WHO}'s {HAT} elimination project. {R}egarding {K}inshasa, it points out the need of regular epidemiologic surveys.},
  keywords = {{H}uman {A}frican {T}rypanosomosis ; {T}setse fly ; {X}enomonitoring ; {K}inshasa ; {A}nimal reservoir ; {O}ne health ; {REPUBLIQUE} {DEMOCRATIQUE} {DU} {CONGO} ; {KINSHASA}},
  booktitle = {},
  journal = {{I}nfection {G}enetics and {E}volution},
  volume = {77},
  numero = {},
  pages = {art. 104095 [5 p.]},
  ISSN = {1567-1348},
  year = {2020},
  DOI = {10.1016/j.meegid.2019.104095},
  URL = {https://www.documentation.ird.fr/hor/fdi:010077730},
}