Sekour S. auteur aut IRD Bekki A. auteur aut IRD Bouchita Z. auteur aut IRD Vogel T.M. auteur aut IRD Navarro Elisabeth auteur aut IRD text journalArticle eng text/pdf reformatted digital access The use of autochtonious bacterial strains is a valuable bioremediation strategy for cleaning the environment from hydrocarbon pollutants. The isolation, selection and identification of hydrocarbon-degrading bacteria is therefore crucial for obtaining the most promising strains for decontaminate a specific site. In this study, two different media, a minimal medium supplemented with petroleum and with oil refinery sludge as sole carbon source, were used for the isolation of native hydrocarbon-degrading bacterial strains from crude oil contaminated soils and oil refinery sludges which allowed isolation of fifty-eight strains.The evalution of diversity of twenty-two bacterials isolates reveled a dominance of the phylum Proteobacteria (20/22 strains), with a unique class of Alphaproteobacteria, the two remaining strains belong to the phylum Actinobacteria. Partial 16S rRNA gene sequencing performed on isolates showed high level of identity with known sequences. Strains were affiliated to Sinorhizobium, Promicromonospora, Novosphingobium, Georgenia, Ancylobacter, Roseomonas, Hansschlegelia and Tistrella genera. Research for the genes that encoding for degradation enzymes in isolated genera genome data deposited in Genbank reveled the presence of degradation gene in three species Sinorhizobium meliloti, Novosphingobium panipatenseand Tistrella mobilis. specialized ALGERIE 084 021 038 9 1 70-77 2019 1338-5178 https://www.documentation.ird.fr/hor/fdi:010077608 10.15414/jmbfs.2019.9.1.70-77 1338-5178 [F B010077608] https://www.documentation.ird.fr/hor/fdi:010077608 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers20-02/010077608.pdf IRD - Base Horizon / Pleins textes 2020-03-04 2025-02-24 fdi:010077608 fre