@article{fdi:010077498,
  title = {{E}xposure of pineapple shoot tips to liquid nitrogen and cryostorage do not affect the histological status of regenerated plantlets},
  author = {{V}illalobos-{O}livera, {A}. and {N}apoles, {L}. and {M}endoza, {J}. {R}. and {E}scalante, {D}. and {M}artinez, {J}. and {C}oncepcion, {O}. and {Z}evallos, {B}. {E}. and {M}artinez-{M}ontero, {M}. {E}. and {C}ejas, {I}. and {E}ngelmann, {F}lorent and {V}icente, {O}. and {L}orenzo, {J}. {C}.},
  editor = {},
  language = {{ENG}},
  abstract = {{C}onservation of pineapple ({A}nanas comosus {L}. {M}err.) genetic resources - including cryopreservation in liquid {N}-2 at -196 degrees {C} - is essential for future breeding programmes to develop new varieties with improved agronomic performance. {H}owever, the potentially deleterious effects of cryopreservation on subsequent plant regrowth should be evaluated before large-scale development of cryobanks is implemented. {T}his paper describes the histological analysis of pineapple plantlets regenerated from cryopreserved shoot tips. {T}wo controls were included in the study: i) conventional micropropagation-derived plantlets, and ii) plants from shoot tips subjected to pre-cryostorage conditioning treatments but never exposed to liquid {N}-2. {H}istological studies of roots, leaves and stems were conducted after 45 days of hardening. {N}o statistically significant differences with the controls were observed in any of the histological parameters evaluated, which supports the practical value of cryopreservation of pineapple germplasm.},
  keywords = {{A}nanas comosus {L}. {M}err. ; cryopreservation ; ex situ conservation ; genetic resources ; histology},
  booktitle = {},
  journal = {{R}omanian {B}iotechnological {L}etters},
  volume = {24},
  numero = {6},
  pages = {1061--1066},
  ISSN = {1224-5984},
  year = {2019},
  DOI = {10.25083/rbl/24.6/1061.1066},
  URL = {https://www.documentation.ird.fr/hor/fdi:010077498},
}