Villalobos-Olivera A., Napoles L., Mendoza J. R., Escalante D., Martinez J., Concepcion O., Zevallos B. E., Martinez-Montero M. E., Cejas I., Engelmann Florent, Vicente O., Lorenzo J. C. (2019). Exposure of pineapple shoot tips to liquid nitrogen and cryostorage do not affect the histological status of regenerated plantlets. Romanian Biotechnological Letters, 24 (6), p. 1061-1066. ISSN 1224-5984.
Titre du document
Exposure of pineapple shoot tips to liquid nitrogen and cryostorage do not affect the histological status of regenerated plantlets
Villalobos-Olivera A., Napoles L., Mendoza J. R., Escalante D., Martinez J., Concepcion O., Zevallos B. E., Martinez-Montero M. E., Cejas I., Engelmann Florent, Vicente O., Lorenzo J. C.
Source
Romanian Biotechnological Letters, 2019,
24 (6), p. 1061-1066 ISSN 1224-5984
Conservation of pineapple (Ananas comosus L. Merr.) genetic resources - including cryopreservation in liquid N-2 at -196 degrees C - is essential for future breeding programmes to develop new varieties with improved agronomic performance. However, the potentially deleterious effects of cryopreservation on subsequent plant regrowth should be evaluated before large-scale development of cryobanks is implemented. This paper describes the histological analysis of pineapple plantlets regenerated from cryopreserved shoot tips. Two controls were included in the study: i) conventional micropropagation-derived plantlets, and ii) plants from shoot tips subjected to pre-cryostorage conditioning treatments but never exposed to liquid N-2. Histological studies of roots, leaves and stems were conducted after 45 days of hardening. No statistically significant differences with the controls were observed in any of the histological parameters evaluated, which supports the practical value of cryopreservation of pineapple germplasm.
Plan de classement
Sciences du monde végétal [076]
;
Biotechnologies [084]
