@article{fdi:010077455,
  title = {{CRISPR} elements provide a new framework for the genealogy of the citrus canker pathogen {X}anthomonas citri pv. citri},
  author = {{J}eong, {K}wanho and {M}unoz-{B}odnar, {A}. and {R}ojas, {N}. {A}. and {P}oulin, {L}. and {R}odriguez-{R}, {L}. {M}. and {G}agnevin, {L}. and {V}erniere, {C}. and {P}ruvost, {O}. and {K}oebnik, {R}alf},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground {X}anthomonads are an important clade of {G}ram-negative bacteria infecting a plethora of economically important host plants, including citrus. {K}nowledge about the pathogen's diversity and population structure are prerequisite for epidemiological surveillance and efficient disease management. {R}apidly evolving genetic loci, such as {C}lustered {R}egularly {I}nterspaced {S}hort {P}alindromic {R}epeats ({CRISPR}), are of special interest to develop new molecular typing tools. {R}esults {W}e analyzed {CRISPR} loci of 56 {X}anthomonas citri pv. citri strains of world-wide origin, a regulated pathogen causing {A}siatic citrus canker in several regions of the world. {W}ith one exception, 23 unique sequences built up the repertoire of spacers, suggesting that this set of strains originated from a common ancestor that already harbored these 23 spacers. {O}ne isolate originating from {P}akistan contained a string of 14 additional, probably more recently acquired spacers indicating that this genetic lineage has or had until recently the capacity to acquire new spacers. {C}omparison of {CRISPR} arrays with previously obtained molecular typing data, such as amplified fragment length polymorphisms ({AFLP}), variable-number of tandem-repeats ({VNTR}) and genome-wide single-nucleotide polymorphisms ({SNP}), demonstrated that these methods reveal similar evolutionary trajectories. {N}otably, genome analyses allowed to generate a model for {CRISPR} array evolution in {X}. citri pv. citri, which provides a new framework for the genealogy of the citrus canker pathogen. {C}onclusions {CRISPR}-based typing will further improve the accuracy of the genetic identification of {X}. citri pv. citri outbreak strains in molecular epidemiology analyses, especially when used concomitantly with another genotyping method.},
  keywords = {{M}olecular typing ; {G}enetic diversity ; {C}lustered regularly interspaced ; short palindromic repeats ; {V}ariable numbers of tandem repeats ; {S}poligotyping ; {E}pidemiology ; {P}hylogeny ; {E}volution ; {X}anthomonas citri pv citri},
  booktitle = {},
  journal = {{BMC} {G}enomics},
  volume = {20},
  numero = {1},
  pages = {art. 917 [19 p.]},
  ISSN = {1471-2164},
  year = {2019},
  DOI = {10.1186/s12864-019-6267-z},
  URL = {https://www.documentation.ird.fr/hor/fdi:010077455},
}