@article{fdi:010077417,
  title = {{D}isentangling {T}rypanosoma cruzi transmission cycle dynamics through the identification of blood meal sources of natural populations of {T}riatoma dimidiata in {Y}ucatan, {M}exico},
  author = {{M}oo-{M}illan, {J}. {I}. and {A}rnal, {A}. and {P}erez-{C}arrillo, {S}. and {H}ernandez-{A}ndrade, {A}. and {R}amirez-{S}ierra, {M}. {J}. and {R}osado-{V}allado, {M}. and {D}umonteil, {E}. and {W}aleckx, {E}tienne},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground {I}n the {Y}ucatan {P}eninsula, {M}exico, {T}riatoma dimidiata is the main vector of {T}rypanosoma cruzi, the causative agent of {C}hagas disease. {L}ittle effort has been made to identify blood meal sources of {T}. dimidiata in natural conditions in this region, although this provides key information to disentangle {T}. cruzi transmission cycles and dynamics and guide the development of more effective control strategies. {W}e identified the blood meals of a large sample of {T}. dimidiata bugs collected in different ecotopes simultaneously with the assessment of bug infection with {T}. cruzi, to disentangle the dynamics of {T}. cruzi transmission in the region. {M}ethods {A} sample of 248 {T}. dimidiata bugs collected in three rural villages and in the sylvatic habitat surrounding these villages was used. {DNA} from each bug midgut was extracted and bug infection with {T}. cruzi was assessed by {PCR}. {F}or blood meal identification, we used a molecular assay based on cloning and sequencing following {PCR} amplification with vertebrate universal primers, and allowing the detection of multiple blood meals in a single bug. {R}esults {O}verall, 28.7% of the bugs were infected with {T}. cruzi, with no statistical difference between bugs from the villages or from sylvatic ecotopes. {S}ixteen vertebrate species including domestic, synanthropic and sylvatic animals, were identified as blood meal sources for {T}. dimidiata. {H}uman, dog and cow were the three main species identified, in bugs collected in the villages as well as in sylvatic ecotopes. {I}mportantly, dog was highlighted as the main blood meal source after human. {D}og was also the most frequently identified animal together with human within single bugs, and tended to be associated with the infection of the bugs. {C}onclusions {D}og, human and cow were identified as the main mammals involved in the connection of sylvatic and domestic transmission cycles in the {Y}ucatan {P}eninsula, {M}exico. {D}og appeared as the most important animal in the transmission pathway of {T}. cruzi to humans, but other domestic and synanthropic animals, which most were previously reported as important hosts of {T}. cruzi in the region, were evidenced and should be taken into account as part of integrated control strategies aimed at disrupting parasite transmission.},
  keywords = {{T}riatoma dimidiata ; {B}lood feeding sources ; {T}rypanosoma cruzi ; {T}ransmission cycles ; {Y}ucatan ; {E}cohealth ; {O}ne {H}ealth ; {C}hagas disease ; {N}atural populations ; {MEXIQUE} ; {YUCATAN}},
  booktitle = {},
  journal = {{P}arasites and {V}ectors},
  volume = {12},
  numero = {1},
  pages = {art. 572 [11 p.]},
  ISSN = {1756-3305},
  year = {2019},
  DOI = {10.1186/s13071-019-3819-7},
  URL = {https://www.documentation.ird.fr/hor/fdi:010077417},
}