NoeM, a new nodulation gene involved in the biosynthesis of Nod Factors with an open-chain oxidized terminal residue and in the symbiosis with Mimosa pudica

NoeM, a new nodulation gene involved in the biosynthesis of Nod Factors with an open-chain oxidized terminal residue and in the symbiosis with Mimosa pudica Daubech B. auteur aut IRD Poinsot V. auteur aut IRD Klonowska Agnieszka auteur aut IRD Capela D. auteur aut IRD Chaintreuil Clémence auteur aut IRD Moulin Lionel auteur aut IRD Marchetti M. auteur aut IRD Masson-Boivin C. auteur aut IRD text journalArticle eng text/pdf born digital access The beta-rhizobium Cupriavidus taiwanensis is a nitrogen-fixing symbiont of Mimosa pudica. Nod factors produced by this species were previously found to be pentameric chitin-oligomers carrying common C18:1 or C16:0 fatty acyl chains, N-methylated and C-6 carbamoylated on the nonreducing terminal N-acetylglucosamine and sulfated on the reducing terminal residue. Here, we report that, in addition, C. taiwanensis LMG19424 produces molecules where the reducing sugar is open and oxidized. We identified a novel nodulation gene located on the symbiotic plasmid pRalta, called noeM, which is involved in this atypical Nod factor structure. noeM encodes a transmembrane protein bearing a fatty acid hydroxylase domain. This gene is expressed during symbiosis with M. pudica and requires NodD and luteolin for optimal expression. The closest noeM homologs formed a separate phylogenetic clade containing rhizobial genes only, which are located on symbiosis plasmids downstream from a nod box. Corresponding proteins, referred to as NoeM, may have specialized in symbiosis via the connection to the nodulation pathway and the spread in rhizobia. noeM was mostly found in isolates of the Mimoseae tribe, and specifically detected in all tested strains able to nodulate M. pudica. A noeM deletion mutant of C. taiwanensis was affected for the nodulation of M. pudica, confirming the role of noeM in the symbiosis with this legume. specialized Cupriavidus taiwanensis host specificity Mimosa pudica Nod factors nodulation rhizobium-legume symbiosis 084 076 Molecular Plant-Microbe Interactions 32 12 1635-1648 2019 0894-0282 https://www.documentation.ird.fr/hor/fdi:010077364 10.1094/mpmi-06-19-0168-r 0894-0282 [F B010077364] https://www.documentation.ird.fr/hor/fdi:010077364 https://www.documentation.ird.fr/intranet/publi/2019/12/010077364.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2020-01-07 2025-02-24 fdi:010077364 fre