Hamrouni R. auteur aut IRD Molinet J. auteur aut IRD Mitropoulou G. auteur aut IRD Kourkoutas Y. auteur aut IRD Dupuy N. auteur aut IRD Masmoudi A. auteur aut IRD Roussos, Sevastianos, auteur aut IRD text journalArticle text/pdf born digital access Currently, the increasing demand of biopesticides production to replace chemical pesticides which are excessively used has made solid state fermentation (SSF) technology the need of the hour. In spite of advantages, true potential of SSF process has not been fully realized at industrial scale. A fermentation process for 6-pentyl-apyrone (6 PP), conidia, and lytic enzymes (cellulases, lipase, amylase) production by Trichoderma asperellum TF1 was scaled-up from 250 mL flasks and glass Raimbault column packed with 20 g of solid substrates (dry weight) to 5 Kg of solid substrate by using a new plastic single used bioreactor. For column and single used bioreactor, the fermentation was done with the application of humid air during all the process however flasks are not hermetically closed that some oxygen could flow by diffusion. T. asperellum growth was investigated using a mixture of vine shoots, jatropha cake, olive pomace and olive oil as substrate in all systems in parallel at 25 degrees C during 7 days. Overall, the conditions applied on the single used bioreactor resulted in the optimum 6-PP production (7.36 +/- 0.37 mg g DM-1), lipases (38.73 +/- 0.21U/g DM), amylases (15.22 +/- 0.13 U/g DM), and conidia production (8.55 +/- 0.04 x 10(9) conidia/g DM). specialized Solid state fermentation Single used bioreactor Trichoderma asperellum 6-Pentyl-a-pyrone Conidia Lytic enzymes 084 252 art. 109496 [9 ] 2019 0301-4797 https://www.documentation.ird.fr/hor/fdi:010077347 10.1016/j.jenvman.2019.109496 0301-4797 [F B010077347] https://www.documentation.ird.fr/hor/fdi:010077347 https://www.documentation.ird.fr/intranet/publi/2019/12/010077347.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2020-01-07 2025-02-24 fdi:010077347 fre