@article{fdi:010077322,
  title = {{B}road-spectrum resistance to bacterial blight in rice using genome editing},
  author = {{O}liva, {R}. and {J}i, {C}. {H}. and {A}tienza-{G}rande, {G}. and {H}uguet-{T}apia, {J}. {C}. and {P}erez-{Q}uintero, {A}. and {L}i, {T}. and {E}om, {J}. {S}. and {L}i, {C}. {H}. and {N}guyen, {H}. and {L}iu, {B}. and {A}uguy, {F}lorence and {S}ciallano, {C}. and {L}uu, {V}. and {D}ossa, {G}. {S}. and {C}unnac, {S}{\'e}bastien and {S}chmidt, {S}. {M}. and {S}lamet-{L}oedin, {I}. {H}. and {C}ruz, {C}. {V}. and {S}zurek, {B}oris and {F}rommer, {W}. {B}. and {W}hite, {F}. {F}. and {Y}ang, {B}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}acterial blight of rice is an important disease in {A}sia and {A}frica. {T}he pathogen, {X}anthomonas oryzae pv. oryzae ({X}oo), secretes one or more of six known transcription-activator-like effectors ({TAL}es) that bind specific promoter sequences and induce, at minimum, one of the three host sucrose transporter genes {SWEET}11, {SWEET}13 and {SWEET}14, the expression of which is required for disease susceptibility. {W}e used {CRISPR}-{C}as9-mediated genome editing to introduce mutations in all three {SWEET} gene promoters. {E}diting was further informed by sequence analyses of {TAL}e genes in 63 {X}oo strains, which revealed multiple {TAL}e variants for {SWEET}13 alleles. {M}utations were also created in {SWEET}14, which is also targeted by two {TAL}es from an {A}frican {X}oo lineage. {A} total of five promoter mutations were simultaneously introduced into the rice line {K}itaake and the elite mega varieties {IR}64 and {C}iherang-{S}ub1. {P}addy trials showed that genome-edited {SWEET} promoters endow rice lines with robust, broad-spectrum resistance.},
  keywords = {},
  booktitle = {},
  journal = {{N}ature {B}iotechnology},
  volume = {37},
  numero = {11},
  pages = {1344--1350 + 1 p.},
  ISSN = {1087-0156},
  year = {2019},
  DOI = {10.1038/s41587-019-0267-z},
  URL = {https://www.documentation.ird.fr/hor/fdi:010077322},
}