@article{fdi:010077310,
  title = {{U}nravelling the metabolic and hormonal machinery during key steps of somatic embryogenesis : a case study in coffee},
  author = {{A}wada, {R}. and {C}ampa, {C}laudine and {G}ibault, {E}. and {D}echamp, {E}. and {G}eorget, {F}. and {L}epelley, {M}. and {A}bdallah, {C}{\'e}cile and {E}rban, {A}. and {M}artinez-{S}eidel, {F}. and {K}opka, {J}. and {L}egendre, {L}. and {L}eran, {S}. and {C}onejero, {G}. and {V}erdeil, {J}. {L}. and {C}rouzillat, {D}. and {B}reton, {D}. and {B}ertrand, {B}. and {E}tienne, {H}.},
  editor = {},
  language = {{ENG}},
  abstract = {{S}omatic embryogenesis ({SE}) is one of the most promising processes for large-scale dissemination of elite varieties. {H}owever, for many plant species, optimizing {SE} protocols still relies on a trial-and-error approach. {U}sing coffee as a model plant, we report here the first global analysis of metabolome and hormone dynamics aiming to unravel mechanisms regulating cell fate and totipotency. {S}ampling from leaf explant dedifferentiation until embryo development covered 15 key stages. {A}n in-depth statistical analysis performed on 104 metabolites revealed that massive re-configuration of metabolic pathways induced {SE}. {D}uring initial dedifferentiation, a sharp decrease in phenolic compounds and caffeine levels was also observed while auxins, cytokinins and ethylene levels were at their highest. {T}otipotency reached its highest expression during the callus stages when a shut-off in hormonal and metabolic pathways related to sugar and energetic substance hydrolysis was evidenced. {A}bscisic acid, leucine, maltotriose, myo-inositol, proline, tricarboxylic acid cycle metabolites and zeatin appeared as key metabolic markers of the embryogenic capacity. {C}ombining metabolomics with multiphoton microscopy led to the identification of chlorogenic acids as markers of embryo redifferentiation. {T}he present analysis shows that metabolite fingerprints are signatures of cell fate and represent a starting point for optimizing {SE} protocols in a rational way.},
  keywords = {cell imaging ; cell fate ; coffee ; histology ; hormone content ; metabolomics ; somatic embryogenesis ; totipotency},
  booktitle = {},
  journal = {{I}nternational {J}ournal of {M}olecular {S}ciences},
  volume = {20},
  numero = {19},
  pages = {art. 4665 [29 p.]},
  year = {2019},
  DOI = {10.3390/ijms20194665},
  URL = {https://www.documentation.ird.fr/hor/fdi:010077310},
}