@article{fdi:010077147, title = {{I}nfluence of yeast extract enrichment and {P}ycnoporus sanguineus inoculum on the dephenolisation of sugar-cane bagasse for production of second-generation ethanol}, author = {{G}onzalez-{B}autista, {E}. and {A}larcon-{G}utierrez, {E}. and {D}upuy, {N}. and {P}erraud {G}aime, {I}sabelle and {Z}iarelli, {F}. and {F}arnet-da-{S}ilva, {A}. {M}.}, editor = {}, language = {{ENG}}, abstract = {{P}retreatment of sugar-cane bagasse ({SCB}) by laccases from {P}ycnoporus sanguineus under solid-state fermentation ({SSF}) can favour dephenolisation of the substrate prior to ethanol production. {T}o define adequate conditions for this biological pretreatment, an experimental design and surface plot analysis were performed using as independent variables: yeast extract, fungal inoculum and substrate amounts after different incubation times. {L}accase and cellulase activities and chemical characterisation of the substrate by {C}-13 {CP}/{MAS} (cross-polarization at magic angle spinning) {NMR} (nuclear magnetic resonance) were used as responses. {A}fter 60 days of {SSF}, yeast extract enrichment and low amounts of inoculum enhanced dephenolisation of {SCB}. {M}oreover, yeast extract reduced the quantity of crystalline cellulose without strongly promoting cellulases and polysaccharide degradation. {H}igh amounts of inoculum decreased laccase activities and substrate dephenolisation.}, keywords = {{B}iological pretreatment ; {P}henoloxidases ; {S}olid-state {NMR}}, booktitle = {}, journal = {{F}uel}, volume = {260}, numero = {}, pages = {art. 116370 [5 p.]}, ISSN = {0016-2361}, year = {2020}, DOI = {10.1016/j.fuel.2019.116370}, URL = {https://www.documentation.ird.fr/hor/fdi:010077147}, }