@article{fdi:010077113,
  title = {{C}ryopreservation of in vitro-grown shoot tips of the medicinal species {C}leome spinosa ({C}leomaceae) applying vitrification-based techniques},
  author = {{V}ilardo, {A}frm and {M}endonca, {T}. {F}. and {E}ngelmann, {F}lorent and {C}ordeiro, {L}. {S}. and {A}lbarello, {N}. and {S}imoes-{G}urgel, {C}.},
  editor = {},
  language = {{ENG}},
  abstract = {{BACKGROUND}: {F}ew cryopreservation studies have been reported with the genus {C}leome. {D}ue to the use of {C}. spinosa in traditional medicine and its valuable pharmacological potential, the long-term conservation of the species will allow the safe maintenance of its germplasm. {OBJECTIVE}: {T}his study compares two vitrification-based techniques on the cryopreservation of shoot tips of {C}. spinosa. {MATERIALS} {AND} {METHODS}: {T}he effect of sucrose preculture and different vitrification solutions was evaluated using vitrification and {V} {C}ryo-plate techniques. {T}he supplementation of recovery medium with {BAP} was also assessed. {RESULTS}: {T}he {V} {C}ryo-plate proved to be the most efficient technique. {T}reatment of shoot tips with {PVS}2 at 0 degrees {C} resulted in a higher regeneration response after cryopreservation when compared to treatment with {PVS}2 and {PVS}3 at 25 degrees {C}. {T}he highest survival (83.3%) and recovery (76.6%) were achieved for shoot tips exposed to {PVS}2 for 90 min at 0 degrees {C} and recovered on {MS} medium supplemented with 0.5 mg {L}-1 {BAP} for 2 weeks. {CONCLUSION}: {P}lants regenerated from cryopreserved shoot tips maintained their in vitro multiplication capacity and showed a normal phenotypic aspect, demonstrating the efficiency of the cryopreservation protocol.},
  keywords = {{BAP} ; long-term conservation ; {PVS} ; shoot tips ; vitrification ; {V} ; {C}ryo-plate},
  booktitle = {},
  journal = {{C}ryoletters},
  volume = {40},
  numero = {4},
  pages = {237--246},
  ISSN = {0143-2044},
  year = {2019},
  URL = {https://www.documentation.ird.fr/hor/fdi:010077113},
}