@article{fdi:010077045,
  title = {{E}thylene signaling is required for fully functional tension wood in hybrid aspen},
  author = {{S}eyfferth, {C}. and {W}essels, {B}. {A}. and {G}orzsas, {A}. and {L}ove, {J}. {W}. and {R}uggeberg, {M}. and {D}elhomme, {N}. and {V}ain, {T}homas and {A}ntos, {K}. and {T}uominen, {H}. and {S}undberg, {B}. and {F}elten, {J}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}ension wood ({TW}) in hybrid aspen trees forms on the upper side of displaced stems to generate a strain that leads to uplifting of the stem. {TW} is characterized by increased cambial growth, reduced vessel frequency and diameter, and the presence of gelatinous, cellulose-rich ({G}-)fibers with its microfibrils oriented parallel to the fiber cell axis. {K}nowledge remains limited about the molecular regulators required for the development of this special xylem tissue with its characteristic morphological, anatomical, and chemical features. {I}n this study, we use transgenic, ethylene-insensitive ({ETI}) hybrid aspen trees together with time-lapse imaging to show that functional ethylene signaling is required for full uplifting of inclined stems. {X}-ray diffraction and {R}aman microspectroscopy of {TW} in {ETI} trees indicate that, although {G}-fibers form, the cellulose microfibril angle in the {G}-fiber {S}-layer is decreased, and the chemical composition of {S}- and {G}-layers is altered than in wild-type {TW}. {T}he characteristic asymmetric growth and reduction of vessel density is suppressed during {TW} formation in {ETI} trees. {A} genome-wide transcriptome profiling reveals ethylene-dependent genes in {TW}, related to cell division, cell wall composition, vessel differentiation, microtubule orientation, and hormone crosstalk. {O}ur results demonstrate that ethylene regulates transcriptional responses related to the amount of {G}-fiber formation and their properties (chemistry and cellulose microfibril angle) during {TW} formation. {T}he quantitative and qualitative changes in {G}-fibers are likely to contribute to uplifting of stems that are displaced from their original position.},
  keywords = {xylem ; wood ; ethylene ; tension wood ; lignin ; microfibril angle ; {R}aman ; microspectroscopy ; transcriptomics},
  booktitle = {},
  journal = {{F}rontiers in {P}lant {S}cience},
  volume = {10},
  numero = {},
  pages = {art. 1101 [17 p.]},
  ISSN = {1664-462{X}},
  year = {2019},
  DOI = {10.3389/fpls.2019.01101},
  URL = {https://www.documentation.ird.fr/hor/fdi:010077045},
}