King E. auteur aut IRD Wallner A. auteur aut IRD Rimbault Isabelle auteur aut IRD Barrachina C. auteur aut IRD Klonowska Agnieszka auteur aut IRD Moulin Lionel auteur aut IRD Czernic P. auteur aut IRD text journalArticle eng text/pdf reformatted digital access In the context of plant-pathogen and plant-mutualist interactions, the underlying molecular bases associated with host colonization have been extensively studied. However, it is not the case for non-mutualistic beneficial interactions or associative symbiosis with plants. Particularly, little is known about the transcriptional regulations associated with the immune tolerance of plants towards beneficial microbes. In this context, the study of the Burkholderia rice model is very promising to describe the molecular mechanisms involved in associative symbiosis. Indeed, several species of the Burkholderia sensu lato (s.l.) genus can colonize rice tissues and have beneficial effects; particularly, two species have been thoroughly studied: Burkholderia vietnamiensis and Paraburkholderia kururiensis. This study aims to compare the interaction of these species with rice and especially to identify common or specific plant responses. Therefore, we analyzed root colonization of the rice cultivar Nipponbare using DsRed-tagged bacterial strains and produced the transcriptomes of both roots and leaves 7 days after root inoculation. This led us to the identification of a co-expression jasmonic acid (JA)-related network exhibiting opposite regulation in response to the two strains in the leaves of inoculated plants. We then monitored by quantitative polymerase chain reaction (qPCR) the expression of JA-related genes during time course colonization by each strain. Our results reveal a temporal shift in this JA systemic response, which can be related to different colonization strategies of both strains. specialized RNAseq endophyte symbiosis Burkholderia rice jasmonic acid 076 020 10 art. 1141 [20 p.] 2019 1664-462X https://www.documentation.ird.fr/hor/fdi:010077044 10.3389/fpls.2019.01141 1664-462X [F B010077044] https://www.documentation.ird.fr/hor/fdi:010077044 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers19-10/010077044.pdf IRD - Base Horizon / Pleins textes 2019-11-05 2025-02-24 fdi:010077044 fre