Suwanmanee S. auteur aut IRD Mahakhunkijcharoen Y. auteur aut IRD Ampawong S. auteur aut IRD Leaungwutiwong P. auteur aut IRD Missé Dorothée auteur aut IRD Luplertlop N. auteur aut IRD text journalArticle eng text/pdf reformatted digital access Dengue virus (DENV) causes dengue fever, a self-limiting disease that could be fatal due to serious complications. No specific treatment is currently available and the preventative vaccine is only partially protective. To develop a potential drug target for dengue fever, we need to understand its biology and pathogenesis thoroughly. N-myristoyltransferase (NMT) is an N-terminal protein lipidation enzyme that catalyzes the covalent cotranslational attachment of fatty acids to the amino-terminal glycine residue of a number of proteins, leading to the modulation of various signaling molecules. In this study, we investigated the interaction of dengue viral proteins with host NMT and its subsequent effect on DENV. Our bioinformatics, molecular docking, and far-western blotting analyses demonstrated the interaction of viral envelope protein (E) with NMT. The gene expression of NMT was strongly elevated in a dependent manner during the viral replication phase in dendritic cells. Moreover, NMT gene silencing significantly inhibited DENV replication in dendritic cells. Further studies investigating the target cell types of other host factors are suggested. specialized dengue virus envelope protein N-myristoyltransferase1 viral replication 052 020 8 9 art. e831 [13 ] 2019 2045-8827 https://www.documentation.ird.fr/hor/fdi:010077022 10.1002/mbo3.831 2045-8827 [F B010077022] https://www.documentation.ird.fr/hor/fdi:010077022 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers19-10/010077022.pdf IRD - Base Horizon / Pleins textes 2019-11-05 2025-02-24 fdi:010077022 fre