Cassidy-Seyoum S. auteur aut IRD Vongsouvath M. auteur aut IRD Sengvilaipaseuth O. auteur aut IRD Seephonelee M. auteur aut IRD Bharucha T. auteur aut IRD Lamballerie X. de auteur aut IRD Newton P. N. auteur aut IRD Dubot Pérès Audrey auteur aut IRD text journalArticle eng text/pdf reformatted digital access Molecular epidemiological data are key for dengue outbreak characterization and preparedness. However, sparse Dengue virus (DENV) molecular information is available in Laos because of limited resources. In this proof-of-concept study, we evaluated whether DENV1 RNA extracted from rapid diagnostic tests (RDTs) could be amplified and sequenced. The protocol for envelope gene amplification from RNA purified from RDTs was first assessed using viral isolate dilutions then conducted using 14 dengue patient sera. Envelope gene amplification was successful from patient sera with high virus titer, as was sequencing but with lower efficiency. Hence, based on our results, RDTs can be a source of DENV1 RNA for subsequent envelope gene amplification and sequencing. This is a promising tool for collecting molecular epidemiology data from rural dengue-endemic areas. However, further investigations are needed to improve assay efficiency and to assess this tool's level of efficacy on a larger scale in the field. specialized LAOS 052 020 101 2 451-455 2019 0002-9637 https://www.documentation.ird.fr/hor/fdi:010077016 10.4269/ajtmh.18-0831 0002-9637 [F B010077016] https://www.documentation.ird.fr/hor/fdi:010077016 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers19-10/010077016.pdf IRD - Base Horizon / Pleins textes 2019-11-05 2025-02-24 fdi:010077016 fre